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Optical pH sensing using spectral analysis
Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of meas...
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Published in: | Sensors and actuators. B, Chemical Chemical, 2007-12, Vol.128 (1), p.133-137 |
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container_title | Sensors and actuators. B, Chemical |
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creator | Fritzsche, M. Barreiro, C.G. Hitzmann, B. Scheper, T. |
description | Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors. |
doi_str_mv | 10.1016/j.snb.2007.05.041 |
format | article |
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The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2007.05.041</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Actuators ; Aminofluorescein ; Chemometrics ; Detection ; Fluorescence ; Indicators ; pH sensing ; Ratiometric sensors ; Sensors ; Spectra ; Temperature dependence</subject><ispartof>Sensors and actuators. 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B, Chemical</title><description>Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.</description><subject>Actuators</subject><subject>Aminofluorescein</subject><subject>Chemometrics</subject><subject>Detection</subject><subject>Fluorescence</subject><subject>Indicators</subject><subject>pH sensing</subject><subject>Ratiometric sensors</subject><subject>Sensors</subject><subject>Spectra</subject><subject>Temperature dependence</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLw0AQhRdRsFZ_gLceRUic3cnuJniSolYo9KLnJdlMZEuaxJ1U6L83tZ69zDvMezO8T4hbCakEaR62KXdVqgBsCjqFTJ6JmcwtJgjWnosZFEonGYC-FFfMWwDI0MBM3G-GMfiyXQyrBVPHoftc7H8nD-THOG3KrmwPHPhaXDRly3Tzp3Px8fL8vlwl683r2_JpnXhEGJNGFbWpJKJWiuTxvUGlJWlLtZJN4bVBIrQyR6wzqa3CrDJgdeGNparGubg73R1i_7UnHt0usKe2LTvq9-wk5EoWBZp8ssqT1ceeOVLjhhh2ZTxMJnfk4rZu4uKOXBxoN3GZMo-nDE0dvgNFxz5Q56kOcWrs6j78k_4BA-hofw</recordid><startdate>20071212</startdate><enddate>20071212</enddate><creator>Fritzsche, M.</creator><creator>Barreiro, C.G.</creator><creator>Hitzmann, B.</creator><creator>Scheper, T.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope></search><sort><creationdate>20071212</creationdate><title>Optical pH sensing using spectral analysis</title><author>Fritzsche, M. ; Barreiro, C.G. ; Hitzmann, B. ; Scheper, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-f29d6b133522e1307763251e57ed21f9c563ee371833d4157234b60759c67ebd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Actuators</topic><topic>Aminofluorescein</topic><topic>Chemometrics</topic><topic>Detection</topic><topic>Fluorescence</topic><topic>Indicators</topic><topic>pH sensing</topic><topic>Ratiometric sensors</topic><topic>Sensors</topic><topic>Spectra</topic><topic>Temperature dependence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fritzsche, M.</creatorcontrib><creatorcontrib>Barreiro, C.G.</creatorcontrib><creatorcontrib>Hitzmann, B.</creatorcontrib><creatorcontrib>Scheper, T.</creatorcontrib><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fritzsche, M.</au><au>Barreiro, C.G.</au><au>Hitzmann, B.</au><au>Scheper, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optical pH sensing using spectral analysis</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2007-12-12</date><risdate>2007</risdate><volume>128</volume><issue>1</issue><spage>133</spage><epage>137</epage><pages>133-137</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>Spectral analysis by chemometric methods is applied to optical pH sensing with aminofluorescein as fluorescent indicator. This method is superior to the ratiometric dual-wavelength detection when the pH value influences the spectrum in a complex way. In the case of aminofluorescein the range of measurement is extended compared to the ratiometric method. The pH value can be calculated from the fluorescence spectra of the indicator independently of the absolute intensity and of the temperature of the sample. At high indicator concentrations the fluorescence spectra are strongly influenced by inner filter effects and the error of the pH prediction is significantly smaller than in a dilute solution. The reduction of the fluorescence spectra to a few data points shows that the chemometric pH determination does not require high-resolution spectral sensors.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2007.05.041</doi><tpages>5</tpages></addata></record> |
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subjects | Actuators Aminofluorescein Chemometrics Detection Fluorescence Indicators pH sensing Ratiometric sensors Sensors Spectra Temperature dependence |
title | Optical pH sensing using spectral analysis |
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