Micropatterning of protein-functionalized magnetic beads on glass using electrostatic self-assembly

We demonstrate a simple and fast method for self-assembly-driven micropatterning of protein-functionalized magnetic beads on glass. We use positively charged aminosilane micro-patterns as template for selective immobilization of streptavidin-coated beads by electrostatic interactions. We show that a...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2008-06, Vol.132 (2), p.361-367
Main Authors: Sivagnanam, V., Sayah, A., Vandevyver, C., Gijs, M.A.M.
Format: Article
Language:English
Subjects:
Actuators
Beads
Channels
Electrostatic self-assembly
Electrostatics
Glass
Micropatterned magnetic beads
Micropatterning
Self assembly
Streptavidin
Tuning
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Summary:We demonstrate a simple and fast method for self-assembly-driven micropatterning of protein-functionalized magnetic beads on glass. We use positively charged aminosilane micro-patterns as template for selective immobilization of streptavidin-coated beads by electrostatic interactions. We show that addition of a non-ionic surfactant to the bead solution increases the selectivity of the micropatterning process. Streptavidin-coated magnetic beads (1.05 and 2.8 μm in size) are immobilized, with high reproducibility and in a very short processing time (∼30 min), in the form of stripes and dots, both on bare substrates and in situ in microfluidic channels. The arrangement and the number of immobilized beads can be controlled by tuning the aminosilane template size.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2007.09.076