Nanowire-integrated microfluidic devices for facile and reagent-free mechanical cell lysis

Cell lysis is an essential task for the detection of intracellular components. In this work, we introduce novel microfluidic devices integrated with patterned one-dimensional nanostructure arrays for facile and high-throughput mechanical cell lysis. The geometry of the hydrothermally grown ZnO nanow...

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Bibliographic Details
Published in:Lab on a chip 2012-08, Vol.12 (16), p.2914-2921
Main Authors: Kim, Jung, Hong, Jung Woo, Kim, Dong Pyo, Shin, Jennifer H, Park, Inkyu
Format: Article
Language:English
Subjects:
Arrays
Cell Line
Cellular
Devices
Extraction
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Jurkat Cells
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Nanomaterials
Nanostructure
Nanowires
Nanowires - chemistry
Nucleic Acids - analysis
Proteins - analysis
Zinc Oxide - chemistry
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Summary:Cell lysis is an essential task for the detection of intracellular components. In this work, we introduce novel microfluidic devices integrated with patterned one-dimensional nanostructure arrays for facile and high-throughput mechanical cell lysis. The geometry of the hydrothermally grown ZnO nanowires, characterised by sharp tips and high aspect ratios, aids in anchoring the cell and tearing the plasma membrane, enabling simple and highly efficient extraction of cellular proteins and nucleic acids. This method lyses cells more effectively than conventional chemical lysis methods with simpler equipment and a shorter processing time.
ISSN:1473-0197
1473-0189
DOI:10.1039/c2lc40154a