Study of Heparin in Intestinal Ischemia and Reperfusion in Rats: Morphologic and Functional Evaluation

Abstract To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R...

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Published in:Transplantation proceedings 2012-10, Vol.44 (8), p.2300-2303
Main Authors: Ghadie, M.M, Miranda-Ferreira, R, Taha, N.S.A, Maroso, A.S, Moreti, R.J.Z, Andraus, M.P, Zempulski, P, Monteiro, H.P, Simões, M.J, Fagundes, D.J, Caricati-Neto, A, Taha, M.O
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytoprotection
Disease Models, Animal
Electric Stimulation
Enteric Nervous System - drug effects
Enteric Nervous System - physiopathology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal Agents - pharmacology
Gastrointestinal Motility - drug effects
Heparin - pharmacology
Jejunum - blood supply
Jejunum - drug effects
Jejunum - innervation
Jejunum - pathology
Jejunum - physiopathology
Male
Medical sciences
Other diseases. Semiology
Potassium Chloride - pharmacology
Rats
Rats, Wistar
Reperfusion Injury - pathology
Reperfusion Injury - physiopathology
Reperfusion Injury - prevention & control
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
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Summary:Abstract To study whether treatment with heparin (HEP) attenuates intestinal dysfunction caused by ischemia (I) and reperfusion (R), rats were treated with HEP (100 U/kg intravenously) or saline solution (SS) before I (60 min), which was produced by occlusion of the superior mesenteric artery, and R (120 min). After I or I/R, we mounted 2-cm jejunal segment in an organ bath to study neurogenic contractions stimulated by electrical pulses or KCl, using a digital recording system. Thin jejunal slices were stained with hematoxylin and eosin for optical microscopy. Compared with the sham group, jejunal contractions were similar in the I + HEP and the I/R + HEP groups, but reduced in the I + SS and the I/R + SS groups. The jejunal enteric nerves were damaged in the I + SS and the I/R + SS, but not in the I + HEP and the I/R + HEP cohorts. These results suggested that HEP attenuated intestinal dysfunction caused by I and I/R.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2012.07.055