Antioxidant and immunoregulatory activity of different polysaccharide fractions from tuber of Ophiopogon japonicus

• Published in: Carbohydrate polymers 2011-08, Vol.86 (3), p.1273-1280
• Main Authors: Xiong, Shuang li, Li, Anlin, Huang, Ni, Lu, Fei, Hou, Dabin
• Format: Article
• Language: English
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; agarose; Antioxidant activity; Antioxidants; Applied sciences; Biological and medical sciences; Carbohydrates; Column chromatography; Energy metabolism; Exact sciences and technology; Extraction and purification; Fourier transform infrared spectroscopy; free radical scavengers; Free radicals; General and cellular metabolism. Vitamins; Guanylate cyclase; High-performance liquid chromatography; hydroxyl radicals; Immunomodulation; Immunomodulators; Immunoregulation; Immunoregulatory activity; Interleukin 1; Medical sciences; Natural polymers; Nitric oxide; Ophiopogon japonicus; Phagocytes; Pharmacology. Drug treatments; Physicochemistry of polymers; Polysaccharide; Polysaccharides; Spectroscopy; Starch and polysaccharides; Sulfate; sulfates; Tuber; Tubers
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2011.06.025

► Five sulfated polysaccharides were prepared from the tuber of Ophiopogon japonicus. ► Polysaccharide with least of sulfate and hexuronic acid had lower antioxidant ability. ► The other four polysaccharides had stronger antioxidant ability. ► Five polysaccharides remarkably activate macrophage and promote cytokine release. ► The antioxidant and immune-enhancing activities depend on the content of hexuronic acid and sulfate. Four sulfated heteropolysaccharide fractions (OJP-1, OJP-2, OJP-3 and OJP-4) were isolated and purified from the tuber of Ophiopogon japonicus by DEAE-Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. OJP-1, the least sulfated product, was sulfated synthetically to OJP-1S. Their chemical–physical properties were determined by chemical methods, GC, FT-IR spectroscopy, and HPLC. The antioxidant and immunomodulation activities of these fractions were also investigated. The content of hexuronic acid and sulfate were decreased in the order OJP-4>OJP-3>OJP-2>OJP-1 and OJP-1S>OJP-4>OJP-3>OJP-2>OJP-1, respectively. In comparison with OJP-1, other polysaccharides showed stronger 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydroxyl radical scavenging activity. Five polysaccharides also exhibited remarkable macrophage-activating capability by the promotion of phagocytic capacity, energy metabolism rate, NO and interleukin-1 production. It was significantly different between five polysaccharides (POJP-4>OJP-3>OJP-2>OJP-1. Taken together, our results suggested that hexuronic acid and sulfate were effective indicators of antioxidant and immunomodulation activity of polysaccharide.