Caffeine, Theophylline and Theobromine Determinations in Serum, Saliva and Spinal Fluid

A rapid method for simultaneous determination of caffeine and theophylline from the serum, saliva, or spinal fluid of neonates was developed. Fifty-microliter samples were extracted and analyzed by high-pressure liquid chromatography (HPLC) at a low accurately detectable limit of 0.1 mg/liter. The s...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical toxicology 1979-01, Vol.3 (1), p.26-29
Main Authors: Tin, Angela A., Somani, Satu M., Bada, Henrietta S., Khanna, Narinder N.
Format: Article
Language:English
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A rapid method for simultaneous determination of caffeine and theophylline from the serum, saliva, or spinal fluid of neonates was developed. Fifty-microliter samples were extracted and analyzed by high-pressure liquid chromatography (HPLC) at a low accurately detectable limit of 0.1 mg/liter. The sample preparation time was about 40 minutes. Instrumental analysis was performed in less than 10 minutes. Extraction efficiency from repeated analyses was determined to be 95 ± A precolumn was used to increase the life of the analytical column. The EMIT-aad® method was compared to HPLC analysis and found to be less efficient.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/3.1.26