The Schotten-Baumann Reaction as an Aid to the Analysis of Polar Compounds: Application to the Determination of Tris(hydroxymethyl)aminomethane (THAM)

The separation of polar, low molecular weight, water-soluble compounds from specimens prior to quantitation has long been a problem in analytical toxicology. Others have successfully used in situ derivatization, prior to extraction, to resolve this problem (1,3). An application of the Schotten-Bauma...

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Bibliographic Details
Published in:Journal of analytical toxicology 1984-09, Vol.8 (5), p.231-233
Main Authors: Blanke, Steven R., Blanke, Robert V.
Format: Article
Language:English
Subjects:
Analysis
Benzoates
Biological and medical sciences
Chromatography, High Pressure Liquid
General pharmacology
Medical sciences
Methods
Pharmacology. Drug treatments
Reference Standards
Tromethamine - analysis
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Summary:The separation of polar, low molecular weight, water-soluble compounds from specimens prior to quantitation has long been a problem in analytical toxicology. Others have successfully used in situ derivatization, prior to extraction, to resolve this problem (1,3). An application of the Schotten-Baumann reaction leading to a simple extraction of tris(hydroxymethyl)aminomethane (THAM) followed by HPLC is presented. This may be a generic approach to the analysis of other polyhydroxy, water-soluble compounds.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/8.5.231