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Screening and comparison of the immunosuppressive activities of polysaccharides from the stems of Ephedra sinica Stapf
Three polysaccharide fractions A, B and C (PA, PB and PC) were acquired from the stems of Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0 M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hyp...
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| Published in: | Carbohydrate polymers 2011-01, Vol.83 (2), p.787-795 |
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| cites | cdi_FETCH-LOGICAL-c396t-7391edb9f650e544817b9c78c78f75ead52b22c909189e80a795f06c67c3cb363 |
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| container_title | Carbohydrate polymers |
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| creator | Kuang, Haixue Xia, Yonggang Yang, Bingyou Wang, Qiuhong Wang, Yanhong |
| description | Three polysaccharide fractions A, B and C (PA, PB and PC) were acquired from the stems of
Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0
M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hypersensitivity reaction and humoral immune response in vivo. Then PB was further isolated to afford four purified polysaccharides (ESP-B1, ESP-B2, ESP-B3 and ESP-B4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and valuated for mice splenocyte proliferation activity in vitro. Among four purified polysaccharides, ESP-B4 has the highest inhibitory effect on splenocyte proliferation and its branches are extremely important for the expression of the inhibitory effect. The results in our study indicate that PB and ESP-B4 have therapeutic potential for the treatment of autoimmune and atopic diseases due to their immunosuppressive effects. |
| doi_str_mv | 10.1016/j.carbpol.2010.08.056 |
| format | article |
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Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0
M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hypersensitivity reaction and humoral immune response in vivo. Then PB was further isolated to afford four purified polysaccharides (ESP-B1, ESP-B2, ESP-B3 and ESP-B4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and valuated for mice splenocyte proliferation activity in vitro. Among four purified polysaccharides, ESP-B4 has the highest inhibitory effect on splenocyte proliferation and its branches are extremely important for the expression of the inhibitory effect. The results in our study indicate that PB and ESP-B4 have therapeutic potential for the treatment of autoimmune and atopic diseases due to their immunosuppressive effects.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2010.08.056</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Atopy ; Biological and medical sciences ; Carbohydrates ; Carbon ; Chemical composition ; cold ; delayed hypersensitivity ; Ephedra sinica ; Ephedra sinica Stapf ; Exact sciences and technology ; gel chromatography ; Gel-filtration chromatography ; humoral immunity ; Hypersensitivity (delayed) ; Immune response (humoral) ; Immunomodulators ; Immunosuppression ; Immunosuppressive effects ; Ion exchange ; Mahuang ; Medical sciences ; mice ; Natural polymers ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Polysaccharide ; Polysaccharides ; screening ; sodium hydroxide ; Splenocytes ; Starch and polysaccharides</subject><ispartof>Carbohydrate polymers, 2011-01, Vol.83 (2), p.787-795</ispartof><rights>2010 Elsevier Ltd.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-7391edb9f650e544817b9c78c78f75ead52b22c909189e80a795f06c67c3cb363</citedby><cites>FETCH-LOGICAL-c396t-7391edb9f650e544817b9c78c78f75ead52b22c909189e80a795f06c67c3cb363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23473926$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuang, Haixue</creatorcontrib><creatorcontrib>Xia, Yonggang</creatorcontrib><creatorcontrib>Yang, Bingyou</creatorcontrib><creatorcontrib>Wang, Qiuhong</creatorcontrib><creatorcontrib>Wang, Yanhong</creatorcontrib><title>Screening and comparison of the immunosuppressive activities of polysaccharides from the stems of Ephedra sinica Stapf</title><title>Carbohydrate polymers</title><description>Three polysaccharide fractions A, B and C (PA, PB and PC) were acquired from the stems of
Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0
M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hypersensitivity reaction and humoral immune response in vivo. Then PB was further isolated to afford four purified polysaccharides (ESP-B1, ESP-B2, ESP-B3 and ESP-B4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and valuated for mice splenocyte proliferation activity in vitro. Among four purified polysaccharides, ESP-B4 has the highest inhibitory effect on splenocyte proliferation and its branches are extremely important for the expression of the inhibitory effect. The results in our study indicate that PB and ESP-B4 have therapeutic potential for the treatment of autoimmune and atopic diseases due to their immunosuppressive effects.</description><subject>Applied sciences</subject><subject>Atopy</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Carbon</subject><subject>Chemical composition</subject><subject>cold</subject><subject>delayed hypersensitivity</subject><subject>Ephedra sinica</subject><subject>Ephedra sinica Stapf</subject><subject>Exact sciences and technology</subject><subject>gel chromatography</subject><subject>Gel-filtration chromatography</subject><subject>humoral immunity</subject><subject>Hypersensitivity (delayed)</subject><subject>Immune response (humoral)</subject><subject>Immunomodulators</subject><subject>Immunosuppression</subject><subject>Immunosuppressive effects</subject><subject>Ion exchange</subject><subject>Mahuang</subject><subject>Medical sciences</subject><subject>mice</subject><subject>Natural polymers</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Polysaccharide</subject><subject>Polysaccharides</subject><subject>screening</subject><subject>sodium hydroxide</subject><subject>Splenocytes</subject><subject>Starch and polysaccharides</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFkU2L2zAQhk1poem2P6HUl0IvTiV_SNaplGX7AQs9pHsW4_Foo2BLrsYJ7L-vsgm9VggEw_POSI-K4r0UWymk-nzYIqRhidO2Frkm-q3o1ItiI3ttKtm07ctiI2TbVr2S-nXxhvkg8lJSbIrTDhNR8OGxhDCWGOcFkucYyujKdU-ln-djiHxclkTM_kQl4OpPfvXEZyaPfWJA3OfYmEsuxfk5yCvNz8TdsqcxQck-eIRyt8Li3havHExM767nTfHw7e737Y_q_tf3n7df7ytsjFor3RhJ42Cc6gR1bdtLPRjUfd5OdwRjVw91jUYY2RvqBWjTOaFQaWxwaFRzU3y69F1S_HMkXu3sGWmaIFA8spXCZD-dNjKj3QXFFJkTObskP0N6ypA9e7YHe_Vsz56t6G32nHMfryOAESaXIKDnf-G6afMr6jP34cI5iBYes2T7sMuNVP6K1mjRZOLLhaBs5OQpWUZPAWn0iXC1Y_T_uctfwsihNA</recordid><startdate>20110110</startdate><enddate>20110110</enddate><creator>Kuang, Haixue</creator><creator>Xia, Yonggang</creator><creator>Yang, Bingyou</creator><creator>Wang, Qiuhong</creator><creator>Wang, Yanhong</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20110110</creationdate><title>Screening and comparison of the immunosuppressive activities of polysaccharides from the stems of Ephedra sinica Stapf</title><author>Kuang, Haixue ; Xia, Yonggang ; Yang, Bingyou ; Wang, Qiuhong ; Wang, Yanhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-7391edb9f650e544817b9c78c78f75ead52b22c909189e80a795f06c67c3cb363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Applied sciences</topic><topic>Atopy</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Carbon</topic><topic>Chemical composition</topic><topic>cold</topic><topic>delayed hypersensitivity</topic><topic>Ephedra sinica</topic><topic>Ephedra sinica Stapf</topic><topic>Exact sciences and technology</topic><topic>gel chromatography</topic><topic>Gel-filtration chromatography</topic><topic>humoral immunity</topic><topic>Hypersensitivity (delayed)</topic><topic>Immune response (humoral)</topic><topic>Immunomodulators</topic><topic>Immunosuppression</topic><topic>Immunosuppressive effects</topic><topic>Ion exchange</topic><topic>Mahuang</topic><topic>Medical sciences</topic><topic>mice</topic><topic>Natural polymers</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Polysaccharide</topic><topic>Polysaccharides</topic><topic>screening</topic><topic>sodium hydroxide</topic><topic>Splenocytes</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuang, Haixue</creatorcontrib><creatorcontrib>Xia, Yonggang</creatorcontrib><creatorcontrib>Yang, Bingyou</creatorcontrib><creatorcontrib>Wang, Qiuhong</creatorcontrib><creatorcontrib>Wang, Yanhong</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuang, Haixue</au><au>Xia, Yonggang</au><au>Yang, Bingyou</au><au>Wang, Qiuhong</au><au>Wang, Yanhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening and comparison of the immunosuppressive activities of polysaccharides from the stems of Ephedra sinica Stapf</atitle><jtitle>Carbohydrate polymers</jtitle><date>2011-01-10</date><risdate>2011</risdate><volume>83</volume><issue>2</issue><spage>787</spage><epage>795</epage><pages>787-795</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>Three polysaccharide fractions A, B and C (PA, PB and PC) were acquired from the stems of
Ephedra sinica Stapf by sequential extraction with cold water, hot water, and 1.0
M NaOH, respectively. Immunosuppressive activities of PA, PB and PC were investigated by carbon clearance test, delayed-type hypersensitivity reaction and humoral immune response in vivo. Then PB was further isolated to afford four purified polysaccharides (ESP-B1, ESP-B2, ESP-B3 and ESP-B4) by various ion exchange and gel-filtration chromatography. Meanwhile, the purified polysaccharides were subjected to composition analysis and valuated for mice splenocyte proliferation activity in vitro. Among four purified polysaccharides, ESP-B4 has the highest inhibitory effect on splenocyte proliferation and its branches are extremely important for the expression of the inhibitory effect. The results in our study indicate that PB and ESP-B4 have therapeutic potential for the treatment of autoimmune and atopic diseases due to their immunosuppressive effects.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.carbpol.2010.08.056</doi><tpages>9</tpages></addata></record> |
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| ispartof | Carbohydrate polymers, 2011-01, Vol.83 (2), p.787-795 |
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| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Applied sciences Atopy Biological and medical sciences Carbohydrates Carbon Chemical composition cold delayed hypersensitivity Ephedra sinica Ephedra sinica Stapf Exact sciences and technology gel chromatography Gel-filtration chromatography humoral immunity Hypersensitivity (delayed) Immune response (humoral) Immunomodulators Immunosuppression Immunosuppressive effects Ion exchange Mahuang Medical sciences mice Natural polymers Pharmacology. Drug treatments Physicochemistry of polymers Polysaccharide Polysaccharides screening sodium hydroxide Splenocytes Starch and polysaccharides |
| title | Screening and comparison of the immunosuppressive activities of polysaccharides from the stems of Ephedra sinica Stapf |
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