A Direct Determination of Tertiary Amyl Alcohol in Plasma

A method is described for the determination of tertiary amyl alcohol in plasma. The procedure employs direct injection of heparinized plasma, separation by gas-liquid-solid chromatography, and subsequent quantification via selective ion monitoring mass spectrometry. Tertiary amyl alcohol is determin...

Full description

Saved in:
Bibliographic Details
Published in:Journal of analytical toxicology 1978-05, Vol.2 (3), p.83-85
Main Authors: Langvardt, P. W., Braun, W. H.
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method is described for the determination of tertiary amyl alcohol in plasma. The procedure employs direct injection of heparinized plasma, separation by gas-liquid-solid chromatography, and subsequent quantification via selective ion monitoring mass spectrometry. Tertiary amyl alcohol is determined by monitoring fragment ions at m/e 59 and m/e 73. Recoveries averaged 99% ± 9% (±S.D.) with a quantitative detection limit (2.5 × noise) of 0.1 µg/ml. The coefficient of variation for five replicate determinations at various plasma concentrations of tertiary amyl alcohol varied from 3.8% at 160 µg/ml to 8.5% at 0.16 µg/ml. Analyses can be accomplished with as little as 50 µl of blood in approximately 15 minutes.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/2.3.83