Impact of Biological Matrix, Drug Concentration, and Method of Isolation on Detectability and Variability of Retention Index Values in Gas Chromatography

The retention indices of eight basic and neutral drugs extracted from plasma, blood, or liver homogenate by six different methods were calculated using temperature-programmed gas chromatography on OV-1 and SE-30 columns. Two concentrations were applied: 1 and 50 mg/L, respectively. The type of extra...

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Bibliographic Details
Published in:Journal of analytical toxicology 1985-03, Vol.9 (2), p.49-54
Main Authors: Bogusz, Maciej, Wijsbeek, Jaap, Franke, Jan Piet, de Zeeuw, Rokus A., Gierz, Jolanta
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Blood
Chromatography, Gas
Drugs
Gas chromatography
General pharmacology
Humans
Liver
Liver - analysis
Medical sciences
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - blood
Pharmacology. Drug treatments
Solvents
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Summary:The retention indices of eight basic and neutral drugs extracted from plasma, blood, or liver homogenate by six different methods were calculated using temperature-programmed gas chromatography on OV-1 and SE-30 columns. Two concentrations were applied: 1 and 50 mg/L, respectively. The type of extraction had great influence on recovery and analytical background. The retention indices tended to be slightly concentration-dependent in that they were somewhat lower at higher drug concentration. Neither the biological sample nor the type of extraction exerted significant influence on scatter and mean value of the retention indices.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/9.2.49