Efficient production of cellulase and xylanase by anaerobic rumen microbial flora grown on wheat straw

Rumen bacteria, fungi and protozoa possess a wide range of enzymes which are capable of hydroryzing most of the structural polysaccharides of the plant cell walls. In the study, the activities of cellulolytic and xylartolytic enzymes produced by anaerobic rumen flora were investigated. Mixed ruminal...

Full description

Saved in:
Bibliographic Details
Published in:African journal of agricultural research 2011-06, Vol.6 (12), p.2711-2714
Main Authors: Bahramian, S, Chamani, M, Azin, M, Gerami, A
Format: Article
Language:English
Subjects:
Anaerobic microorganisms
Protozoa
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rumen bacteria, fungi and protozoa possess a wide range of enzymes which are capable of hydroryzing most of the structural polysaccharides of the plant cell walls. In the study, the activities of cellulolytic and xylartolytic enzymes produced by anaerobic rumen flora were investigated. Mixed ruminal microorganisms were grown on wheat straw in anaerobic liquid medium that have been purged with oxygen-free CO sub(2) and allowed to ferment for 0, 24, 48, 72 and 120 h at 39 degree C. Then the supernatants of each culture were analyzed for cellulase and xylanase activities with dinitrosalicylic acid (DNS) method. Results showed that optimum time for maximum activity of both enzymes is 48 h incubation at 39 degree C and at each time pointed, xylanase activity was much higher than cellulase activity.
ISSN:1991-637X
1991-637X
DOI:10.5897/AJAR10.029