MiR-21 is Enriched in the RNA-Induced Silencing Complex and Targets COL4A1 in Human Granulosa Cell Lines

MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and f...

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Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2012-10, Vol.19 (10), p.1030-1040
Main Authors: Mase, Yuri, Ishibashi, Osamu, Ishikawa, Tomoko, Takizawa, Takami, Kiguchi, Kazushige, Ohba, Takashi, Katabuchi, Hidetaka, Takeshita, Toshiyuki, Takizawa, Toshihiro
Format: Article
Language:English
Subjects:
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Basement membranes
Carboxypeptidases - metabolism
Cell Line
Collagen Type IV - biosynthesis
Data processing
Embryology
Female
Gene Expression Profiling
Granulosa cells
Granulosa Cells - metabolism
Guanylate cyclase
Humans
Medicine & Public Health
MicroRNAs - analysis
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
mRNA
Obstetrics/Perinatology/Midwifery
Original Articles
Reproductive Medicine
RNA-induced silencing complex
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Summary:MicroRNAs (miRNAs) are noncoding small RNAs that play important roles in a variety of physiological and pathological events. In this study, we performed large-scale profiling of EIF2C2-bound miRNAs in 3 human granulosa-derived cell lines (ie, KGN, HSOGT, and GC1a) by high-throughput sequencing and found that miR-21 accounted for more than 80% of EIF2C2-bound miRNAs, suggesting that it was enriched in the RNA-induced silencing complex (RISC) and played a functional role in human granulosa cell (GC) lines. We also found high expression levels of miR-21 in primary human GCs. Assuming that miR-21 target mRNAs are enriched in RISC, we performed cDNA cloning of EIF2C2-bound mRNAs in KGN cells. We identified COL4A1 mRNA as a miR-21 target in the GC lines. These data suggest that miR-21 is involved in the regulation of the synthesis of COL4A1, a component of the basement membrane surrounding the GC layer and granulosa-embedded extracellular structure.
ISSN:1933-7191
1933-7205
DOI:10.1177/1933719112442245