An Enzyme-Linked Immunosorbent Assay (ELISA) for Methylphenidate (Ritalin®) in Urine

A direct enyzme-linked immunosorbent assay (ELISA) for urinary immunoreactive methylphenidate (Ritalin), in which a standard 96-well microtiter plate is used, is described. For this ELISA, a methylphenidate-thyroglobulin conjugate is immobilized to the microtiter plate and competes with methylphenid...

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Bibliographic Details
Published in:Journal of analytical toxicology 2003-09, Vol.27 (6), p.342-345
Main Authors: Lewis, Mark G., Lewis, John G., Elder, Peter A., Moore, Grant A.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Cattle
Central Nervous System Stimulants - immunology
Central Nervous System Stimulants - urine
Color
Drug abuse
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
General pharmacology
Humans
Immunoglobulin G
Immunoglobulin G - immunology
Immunosorbents
Medical sciences
Methylphenidate
Methylphenidate - immunology
Methylphenidate - urine
Peroxidase - immunology
Pharmacology. Drug treatments
Reproducibility of Results
Sensitivity and Specificity
Sheep
Thyroglobulin - metabolism
Urinalysis
Urine
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Description
Summary:A direct enyzme-linked immunosorbent assay (ELISA) for urinary immunoreactive methylphenidate (Ritalin), in which a standard 96-well microtiter plate is used, is described. For this ELISA, a methylphenidate-thyroglobulin conjugate is immobilized to the microtiter plate and competes with methylphenidate in the standard or urine sample for antibody-binding sites. After washing, the sheep methylphenidate antibody bound to immobilized methylphenidate is detected with peroxidase-labelled goat antisheep IgG. Following a further wash, tetramethylbenzidine is added, color is developed, and the plate is read at 450 nm on an ELISA plate reader. This method is unaffected by drugs of abuse and is suitable for routine use in the toxicology laboratory.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/27.6.342