Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney

Background Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI. Methods Adipose-derived mesenchy...

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Bibliographic Details
Published in:Clinical and experimental nephrology 2012-10, Vol.16 (5), p.679-689
Main Authors: Furuichi, Kengo, Shintani, Hidemi, Sakai, Yoshio, Ochiya, Takahiro, Matsushima, Kouji, Kaneko, Shuichi, Wada, Takashi
Format: Article
Language:English
Subjects:
Acute Kidney Injury - pathology
Acute Kidney Injury - therapy
Adipose Tissue - cytology
Animals
Cell Differentiation
Cell Separation
Chemokines - biosynthesis
Cytokines - biosynthesis
Kidney - pathology
Kidney Tubular Necrosis, Acute - prevention & control
Lung - pathology
Male
Medicine
Medicine & Public Health
Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells
Mice
Mice, Inbred C57BL
Nephrology
Original Article
Reperfusion Injury - pathology
Reperfusion Injury - therapy
Urology
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Summary:Background Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI. Methods Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10 5  cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2. Results The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression. Conclusion Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.
ISSN:1342-1751
1437-7799
DOI:10.1007/s10157-012-0614-6