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Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney
Background Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI. Methods Adipose-derived mesenchy...
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| Published in: | Clinical and experimental nephrology 2012-10, Vol.16 (5), p.679-689 |
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| container_end_page | 689 |
| container_issue | 5 |
| container_start_page | 679 |
| container_title | Clinical and experimental nephrology |
| container_volume | 16 |
| creator | Furuichi, Kengo Shintani, Hidemi Sakai, Yoshio Ochiya, Takahiro Matsushima, Kouji Kaneko, Shuichi Wada, Takashi |
| description | Background
Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Methods
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10
5
cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
Results
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Conclusion
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury. |
| doi_str_mv | 10.1007/s10157-012-0614-6 |
| format | article |
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Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Methods
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10
5
cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
Results
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Conclusion
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.</description><identifier>ISSN: 1342-1751</identifier><identifier>EISSN: 1437-7799</identifier><identifier>DOI: 10.1007/s10157-012-0614-6</identifier><identifier>PMID: 22398959</identifier><identifier>CODEN: CENPFV</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Acute Kidney Injury - pathology ; Acute Kidney Injury - therapy ; Adipose Tissue - cytology ; Animals ; Cell Differentiation ; Cell Separation ; Chemokines - biosynthesis ; Cytokines - biosynthesis ; Kidney - pathology ; Kidney Tubular Necrosis, Acute - prevention & control ; Lung - pathology ; Male ; Medicine ; Medicine & Public Health ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; Mice ; Mice, Inbred C57BL ; Nephrology ; Original Article ; Reperfusion Injury - pathology ; Reperfusion Injury - therapy ; Urology</subject><ispartof>Clinical and experimental nephrology, 2012-10, Vol.16 (5), p.679-689</ispartof><rights>Japanese Society of Nephrology 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-553f4ce6a96b4e18d7431d00c340461b607afc5c9f1fcdc93a5fe65339635ea93</citedby><cites>FETCH-LOGICAL-c480t-553f4ce6a96b4e18d7431d00c340461b607afc5c9f1fcdc93a5fe65339635ea93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22398959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furuichi, Kengo</creatorcontrib><creatorcontrib>Shintani, Hidemi</creatorcontrib><creatorcontrib>Sakai, Yoshio</creatorcontrib><creatorcontrib>Ochiya, Takahiro</creatorcontrib><creatorcontrib>Matsushima, Kouji</creatorcontrib><creatorcontrib>Kaneko, Shuichi</creatorcontrib><creatorcontrib>Wada, Takashi</creatorcontrib><title>Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney</title><title>Clinical and experimental nephrology</title><addtitle>Clin Exp Nephrol</addtitle><addtitle>Clin Exp Nephrol</addtitle><description>Background
Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Methods
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10
5
cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
Results
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Conclusion
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.</description><subject>Acute Kidney Injury - pathology</subject><subject>Acute Kidney Injury - therapy</subject><subject>Adipose Tissue - cytology</subject><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Separation</subject><subject>Chemokines - biosynthesis</subject><subject>Cytokines - biosynthesis</subject><subject>Kidney - pathology</subject><subject>Kidney Tubular Necrosis, Acute - prevention & control</subject><subject>Lung - pathology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Nephrology</subject><subject>Original Article</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - therapy</subject><subject>Urology</subject><issn>1342-1751</issn><issn>1437-7799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kE1OHDEQhS1EFH6SA2SDWmKTjaGq_ddeRogAEhIboiwtj7sMPUx3T-xppNlxB27ISfBoCIoiUZsqub56fnqMfUM4QQBzmhFQGQ5Yc9Aoud5h-yiF4cZYu1tmIWuORuEeO8h5DgCNVfYz26trYTfjPvt9HiOFVa7GWPm2W46ZeEupe6S26inTEO7XvV9UgRaLAg1Vl8M99Z1_eXpOtKQUp9xtnof5lNalVQ9dO9D6C_sU_SLT17d-yH79PL89u-TXNxdXZz-ueZANrLhSIspA2ls9k4RNa6TAFiAICVLjTIPxMahgI8bQBiu8iqSVEFYLRd6KQ_Z9q7tM45-J8sr1xWEx6wcap-ywVKO0gqagx_-h83FKQ3HnsG4UGBAaCoVbKqQx50TRLVPX-7R2CG6Tutum7krqbpO60-Xm6E15mvXUvl_8jbkA9RbIZTXcUfrn6w9VXwFNYY27</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Furuichi, Kengo</creator><creator>Shintani, Hidemi</creator><creator>Sakai, Yoshio</creator><creator>Ochiya, Takahiro</creator><creator>Matsushima, Kouji</creator><creator>Kaneko, Shuichi</creator><creator>Wada, Takashi</creator><general>Springer Japan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20121001</creationdate><title>Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney</title><author>Furuichi, Kengo ; Shintani, Hidemi ; Sakai, Yoshio ; Ochiya, Takahiro ; Matsushima, Kouji ; Kaneko, Shuichi ; Wada, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-553f4ce6a96b4e18d7431d00c340461b607afc5c9f1fcdc93a5fe65339635ea93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acute Kidney Injury - pathology</topic><topic>Acute Kidney Injury - therapy</topic><topic>Adipose Tissue - cytology</topic><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cell Separation</topic><topic>Chemokines - biosynthesis</topic><topic>Cytokines - biosynthesis</topic><topic>Kidney - pathology</topic><topic>Kidney Tubular Necrosis, Acute - prevention & control</topic><topic>Lung - pathology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Nephrology</topic><topic>Original Article</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - therapy</topic><topic>Urology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furuichi, Kengo</creatorcontrib><creatorcontrib>Shintani, Hidemi</creatorcontrib><creatorcontrib>Sakai, Yoshio</creatorcontrib><creatorcontrib>Ochiya, Takahiro</creatorcontrib><creatorcontrib>Matsushima, Kouji</creatorcontrib><creatorcontrib>Kaneko, Shuichi</creatorcontrib><creatorcontrib>Wada, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furuichi, Kengo</au><au>Shintani, Hidemi</au><au>Sakai, Yoshio</au><au>Ochiya, Takahiro</au><au>Matsushima, Kouji</au><au>Kaneko, Shuichi</au><au>Wada, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney</atitle><jtitle>Clinical and experimental nephrology</jtitle><stitle>Clin Exp Nephrol</stitle><addtitle>Clin Exp Nephrol</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>16</volume><issue>5</issue><spage>679</spage><epage>689</epage><pages>679-689</pages><issn>1342-1751</issn><eissn>1437-7799</eissn><coden>CENPFV</coden><abstract>Background
Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI.
Methods
Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10
5
cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2.
Results
The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression.
Conclusion
Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>22398959</pmid><doi>10.1007/s10157-012-0614-6</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1342-1751 |
| ispartof | Clinical and experimental nephrology, 2012-10, Vol.16 (5), p.679-689 |
| issn | 1342-1751 1437-7799 |
| language | eng |
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| source | Springer Nature |
| subjects | Acute Kidney Injury - pathology Acute Kidney Injury - therapy Adipose Tissue - cytology Animals Cell Differentiation Cell Separation Chemokines - biosynthesis Cytokines - biosynthesis Kidney - pathology Kidney Tubular Necrosis, Acute - prevention & control Lung - pathology Male Medicine Medicine & Public Health Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells Mice Mice, Inbred C57BL Nephrology Original Article Reperfusion Injury - pathology Reperfusion Injury - therapy Urology |
| title | Effects of adipose-derived mesenchymal cells on ischemia–reperfusion injury in kidney |
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