Neural stimulation on human bone marrow-derived mesenchymal stem cells by extremely low frequency electromagnetic fields

Adult stem cells are considered multipotent. Especially, human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the bi...

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Bibliographic Details
Published in:Biotechnology progress 2012-09, Vol.28 (5), p.1329-1335
Main Authors: Cho, Hyunjin, Seo, Young-Kwon, Yoon, Hee-Hoon, Kim, Soo-Chan, Kim, Sung-Min, Song, Kye-Yong, Park, Jung-Keug
Format: Article
Language:English
Subjects:
Basic Helix-Loop-Helix Transcription Factors - genetics
Basic Helix-Loop-Helix Transcription Factors - metabolism
Biological and medical sciences
Biotechnology
Bone Marrow Cells - chemistry
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
bone marrow mesenchymal stem cells
Cell Differentiation
Cell Line
Cell Proliferation
Electromagnetic Fields
extremely low frequency electromagnetic field
Fundamental and applied biological sciences. Psychology
Gene Expression
Humans
Mesenchymal Stromal Cells - chemistry
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
neural cells
Neural Stem Cells - chemistry
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
tau Proteins - genetics
tau Proteins - metabolism
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Summary:Adult stem cells are considered multipotent. Especially, human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the biological effects of EMFs. hBM‐MSCs are weak and sensitive pluripotent stem cells, therefore extremely low frequency‐electromagnetic fields (ELF‐EMFs) could be affect the changes of biological functions within the cells. In our experiments, ELF‐EMFs inhibited the growth of hBM‐MSCs in 12 days exposure. Their gene level was changed and expression of the neural stem cell marker like nestin was decreased but the neural cell markers like MAP2, NEUROD1, NF‐L, and Tau were induced. In immunofluorescence study, we confirmed the expression of each protein of neural cells. And also both oligodendrocyte and astrocyte related proteins like O4 and GFAP were expressed by ELF‐EMFs. We suggest that EMFs can induce neural differentiation in BM‐MSCs without any chemicals or differentiation factors. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.1607