The state of the art for pluripotent stem cells derivation in domestic ungulates

Since the successful isolation, characterization and long-term culture of embryonic stem cells (ESCs) from mice in the early 1980s and from humans a decade later, considerable effort has been made to establish ESCs lines from livestock. The derivation of validated ESCs lines is a necessary step if t...

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Bibliographic Details
Published in:Theriogenology 2012-11, Vol.78 (8), p.1749-1762
Main Authors: Malaver-Ortega, Luis Fernando, Sumer, Huseyin, Liu, Jun, Verma, Paul J.
Format: Article
Language:English
Subjects:
Animals
Bovine
Cattle - embryology
Cell Differentiation
Cell Separation - veterinary
Cells, Cultured
Cloning, Organism - veterinary
embryonic stem cells
Embryonic Stem Cells - cytology
ESC
Goats - embryology
humans
Induced Pluripotent Stem Cells - cytology
iPSC
livestock
livestock production
mice
Ovine
Pluripotent Stem Cells - cytology
Sheep - embryology
somatic cells
Stem cells
Sus scrofa - embryology
transcription factors
transgenic animals
Ungulates
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Summary:Since the successful isolation, characterization and long-term culture of embryonic stem cells (ESCs) from mice in the early 1980s and from humans a decade later, considerable effort has been made to establish ESCs lines from livestock. The derivation of validated ESCs lines is a necessary step if the generation of economically relevant transgenic animals is to be achieved. However, this is still elusive, as the isolation of true ESCs lines for livestock has not been accomplished to date. It has been demonstrated that by forced expression of a defined set of transcription factors, it is possible to reprogram somatic cells to cells that closely resemble an ES-like state. These cells were termed induced pluripotent stem cells (iPSCs). We introduce the basic concepts relating to stem cell biology and give an overview of the various attempts to isolate and generate pluripotent stem cells (PSCs) from species relevant to livestock production. Further, we point out the issues to be addressed and hurdles to be overcome to realize the promise of stem cells in agriculture.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2012.03.031