Nutritional requirements of the BY series of Saccharomyces cerevisiae strains for optimum growth

Abstract Among the vast variety of Saccharomyces cerevisiae strains, the BY family is particularly important because the widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum gro...

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Bibliographic Details
Published in:FEMS yeast research 2012-11, Vol.12 (7), p.796-808
Main Authors: Hanscho, Michael, Ruckerbauer, David E., Chauhan, Neha, Hofbauer, Harald F., Krahulec, Stefan, Nidetzky, Bernd, Kohlwein, Sepp D., Zanghellini, Juergen, Natter, Klaus
Format: Article
Language:English
Subjects:
amino acid
Amino acids
Amino Acids - metabolism
auxotrophy
Cell size
Clonal deletion
Culture Media - chemistry
Dietary supplements
flux balance analysis
Gene deletion
Glucose
Glucose - metabolism
Glutamic acid
Growth conditions
Growth rate
Inositol
Inositol - metabolism
Lipid Metabolism
Mycology - methods
Phenylalanine
Saccharomyces cerevisiae
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Serine
Threonine
Yeast
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Summary:Abstract Among the vast variety of Saccharomyces cerevisiae strains, the BY family is particularly important because the widely used deletion collections are based on this background. Here we demonstrate that some standard growth media recipes require substantial modifications to provide optimum growth conditions for auxotrophic BY strains and to avoid growth arrest before glucose is depleted. In addition to the essential supplements that are required to satisfy auxotrophic requirements, we found the four amino acids phenylalanine, glutamic acid, serine, and threonine to be indispensable for optimum growth, despite the fact that BY is ‘prototrophic’ for these amino acids. Interestingly, other widely used S. cerevisiae strains, such as strains of the CEN.PK family, are less sensitive to lack of the described supplements. Furthermore, we found that the concentration of inositol in yeast nitrogen base is too low to support fast proliferation of yeast cultures until glucose is exhausted. Depletion of inositol during exponential growth induces characteristic changes, namely a decrease in glucose uptake and maximum specific growth rate, increased cell size, reduced viability, and accumulation of lipid storage pools. Thus, several of the existing growth media recipes need to be revised to achieve optimum growth conditions for BY-derived strains.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2012.00830.x