Cochlin expression in the rat perilymph during postnatal development

Abstract Conclusions: The changes in the cochlin isoforms in the perilymph may provide important insights to the understanding of cochlin function and the pathogenesis of related inner ear diseases. Objectives: Cochlin is involved in various pathologies of the inner ear. Altered levels of cochlin is...

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Bibliographic Details
Published in:Acta oto-laryngologica 2012-11, Vol.132 (11), p.1134-1139
Main Authors: Shiiba, Kyoko, Shindo, Susumu, Ikezono, Tetsuo, Sekine, Kuwon, Matsumura, Tomohiro, Sekiguchi, Satomi, Yagi, Toshiaki, Okubo, Kimihiro
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
COCH gene
DFNA9
Ear - growth & development
Extracellular Matrix Proteins - metabolism
Medical sciences
Otorhinolaryngology. Stomatology
Perilymph - metabolism
Protein Isoforms - metabolism
Rats
Rats, Wistar
real-time PCR
Real-Time Polymerase Chain Reaction
Western blot
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Summary:Abstract Conclusions: The changes in the cochlin isoforms in the perilymph may provide important insights to the understanding of cochlin function and the pathogenesis of related inner ear diseases. Objectives: Cochlin is involved in various pathologies of the inner ear. Altered levels of cochlin isoforms in developing inner ear tissue were reported previously. The purpose of this study was to elucidate the cochlin isoform expression in the perilymph of rats during postnatal development in relation to Coch gene mRNA expression. Methods: We studied the cochlin isoforms in the rat perilymph during postnatal development by Western blot analysis. Real-time PCR was also performed to elucidate the expression level of Coch mRNA in the developing inner ear of rats. Results: Western blot analysis showed that the expression of p63s in the perilymph was highest on the 12th day after birth (DAB12), the earliest age at which we could identify the perilymphatic space microscopically, and it decreased gradually as the cochlea developed. On the other hand, the expression of Cochlin-tomoprotein (CTP)was lowest on DAB12 and increased gradually up to DAB24. COCH mRNA was detected from DAB3 and gradually increased to DAB15, and then gradually decreased to DAB70.
ISSN:0001-6489
1651-2251
DOI:10.3109/00016489.2012.687456