The nucleotide-binding oligomerization domain-containing-2 ligand muramyl dipeptide enhances phagocytosis and intracellular killing of Escherichia coli K1 by Toll-like receptor agonists in microglial cells

Abstract Increasing the phagocytic activity of microglia could improve the resistance of immunocompromised patients to CNS infections. We studied the microglial responses upon stimulation with the Nod2 ligand muramyl dipeptide (MDP) alone or in combination with a TLR1/2, 3 or 4 agonist. MDP caused a...

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Bibliographic Details
Published in:Journal of neuroimmunology 2012-11, Vol.252 (1), p.16-23
Main Authors: Ribes, Sandra, Adam, Nina, Schütze, Sandra, Regen, Tommy, Redlich, Sandra, Janova, Hana, Borisch, Angela, Hanisch, Uwe-Karsten, Nau, Roland
Format: Article
Language:English
Subjects:
Acetylmuramyl-Alanyl-Isoglutamine - immunology
Acetylmuramyl-Alanyl-Isoglutamine - pharmacology
Adjuvants, Immunologic - pharmacology
Allergy and Immunology
Animals
Antigen presentation
Cells, Cultured
Central nervous system
Cytokines
Cytotoxicity, Immunologic - immunology
Escherichia coli
Escherichia coli - immunology
Escherichia coli Infections - immunology
Flow Cytometry
Immunity, Innate - immunology
Immunocompromised hosts
Infection
Inflammation
Intracellular killing
Ligands
Mice
Mice, Inbred C57BL
Microglia
Microglia - drug effects
Microglia - immunology
Microglial cells
Muramyl dipeptide
Neurology
Nitric oxide
NOD2 protein
Nod2 Signaling Adaptor Protein - immunology
Oligomerization
Phagocytes
Phagocytosis
Phagocytosis - drug effects
Phagocytosis - immunology
TLR1 protein
Toll-like receptors
Toll-Like Receptors - immunology
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Summary:Abstract Increasing the phagocytic activity of microglia could improve the resistance of immunocompromised patients to CNS infections. We studied the microglial responses upon stimulation with the Nod2 ligand muramyl dipeptide (MDP) alone or in combination with a TLR1/2, 3 or 4 agonist. MDP caused a mild release of NO, but induced neither a significant release of pro-inflammatory cytokines nor an expression of molecules associated with professional antigen presentation. Using the Escherichia coli K1 model, microglial pre-stimulation with MDP enhanced bacterial phagocytosis which was strengthened on TLR-pre-stimulated cells. Dual pre-stimulation of Nod2 and TLR1/2 or 4 caused maximal phagocytosis and intracellular killing.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2012.07.012