An X-chromosome RNA-binding motif protein (RBMX) is required for proper kinetochore formation

RBMX is an RNA-binding motif protein identified as a component of purified human metaphase chromosomes. We have analyzed the functional significance of RBMX by electron microscopy (EM) in combination with RNA interference (RNAi). We revealed that depletion of RBMX results in the accumulation of mito...

Full description

Saved in:
Bibliographic Details
Published in:Chromosome Science 2011, Vol.14(1+2), pp.17-21
Main Authors: Lee, Mei Hann, Lin, Linyen, Takata, Hideaki, Morimoto, Akihiro, Uchiyama, Susumu, Matsunaga, Sachihiro, Fukui, Kiichi
Format: Article
Language:English
Subjects:
Chromosomes
Data processing
Differentiation
Electron microscopy
hnRNP G (heterogeneous nuclear ribonucleoprotein G)
kinetochore
Kinetochores
Metaphase
Microtubules
RBMX (RNA-binding motif protein)
RNA interference
RNA-mediated interference
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:RBMX is an RNA-binding motif protein identified as a component of purified human metaphase chromosomes. We have analyzed the functional significance of RBMX by electron microscopy (EM) in combination with RNA interference (RNAi). We revealed that depletion of RBMX results in the accumulation of mitotic cells. EM investigation further showed that RBMX depletion leads to an increase in the fuzzy ball structure that is reminiscent of the prekinetochore. It is most probably due to the result of disruption at early stages of kinetochore maturation, so that kinetochore differentiation into the authentic trilaminar morphology is disturbed. The present EM data illuminated the importance of RBMX in kinetochore formation for subsequent stable microtubules interaction.
ISSN:1344-1051
2185-0852
DOI:10.11352/scr.14.17