Changes in NADPH oxidase mRNA level can be detected in blood at inhaled corticosteroid treated asthmatic children

• Published in: Life sciences (1973) 2012-11, Vol.91 (19-20), p.907-911
• Main Authors: Ökrös, Zsuzsanna, Endreffy, Emoke, Novak, Zoltan, Maroti, Zoltan, Monostori, Peter, Varga, Ilona Sz, Király, Agnes, Turi, Sandor
• Format: Article
• Language: English
• Subjects: Administration, Inhalation; Adolescent; Adrenergic beta-2 Receptor Agonists - administration & dosage; Adrenergic beta-2 Receptor Agonists - pharmacology; Adrenergic beta-2 Receptor Agonists - therapeutic use; agonists; antioxidants; asthma; Asthma - drug therapy; Blood; Bronchial asthma; Case-Control Studies; childhood; children; Dose-Response Relationship, Drug; Drug Therapy, Combination; Female; gene expression; Gene Expression Regulation; Glucocorticoids - administration & dosage; Glucocorticoids - pharmacology; Glucocorticoids - therapeutic use; heme oxygenase (biliverdin-producing); Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Humans; inflammation; Inhaled corticosteroids; isozymes; leukocytes; Leukocytes - metabolism; Male; medical treatment; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; messenger RNA; NADPH oxidase; NADPH Oxidase 2; NADPH Oxidases - genetics; NADPH Oxidases - metabolism; Oxidative Stress; patients; Polymerase Chain Reaction; Protein Carbonylation; quantitative polymerase chain reaction; RNA, Messenger - metabolism; Thiobarbituric Acid Reactive Substances - metabolism; thiobarbituric acid-reactive substances
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2012.08.039

Oxidative stress, observed in the asthmatic airways, is not localized only to the bronchial system. It would be a great advantage to monitor the oxidative stress markers from blood especially in childhood asthma following the inflammation. Our aim was to measure the levels of antioxidants and the oxidatively damaged biomolecules. We were also interested in the gene expression alterations of the free radical source gp91phox subunit (CYBB) of the NADPH oxidase system, and the antioxidant heme oxygenase-1 (HMOX-1) isoenzyme in the blood. Our findings were also examined in the context of medical treatment. Oxidative stress parameters via photometric methods, CYBB and HMOX‐1 expressions via real-time PCR were measured in 58 asthmatic and 30 healthy children. Higher blood thiobarbituric acid reactive substances (TBARS) (p