Molecular characterization and expression profiles of cdc2 and cyclin B during oogenesis and spermatogenesis in green mud crab (Scylla paramamosain)

The maturation promoting factor (MPF) is a key regulator of controlling G2/M phase transition in the meiotic maturation of oocyte and spermatocyte in animals, which is a complex of CDC2 (CDK1) and cyclin B. To better understand the molecular mechanism of oocyte and spermatocyte maturation in mud cra...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2012-11, Vol.163 (3-4), p.292-302
Main Authors: Han, Kunhuang, Dai, Yanbin, Zou, Zhihua, Fu, Mingjun, Wang, Yilei, Zhang, Ziping
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acids
animal ovaries
Animals
Base Sequence
Brachyura - enzymology
Brachyura - genetics
Brachyura - growth & development
Brachyura - metabolism
CDC2 Protein Kinase - chemistry
CDC2 Protein Kinase - genetics
CDC2 Protein Kinase - metabolism
Cloning, Molecular
complementary DNA
crabs
Cyclin B - chemistry
Cyclin B - genetics
Cyclin B - metabolism
cyclins
DNA, Complementary - genetics
Female
Gametogenesis
Gene expression
Gene Expression Regulation, Developmental
genes
in situ hybridization
Male
maturation-promoting factor
messenger RNA
Molecular Sequence Data
nucleotide sequences
oocytes
Oocytes - cytology
Oocytes - metabolism
oogenesis
Oogenesis - genetics
oogonia
ovarian development
Phylogeny
quantitative polymerase chain reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Scylla paramamosain
Sequence Homology, Amino Acid
Sp-cdc2
Sp-cyclin B
spermatogenesis
Spermatogenesis - genetics
Spermatozoa - cytology
Spermatozoa - metabolism
testes
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Summary:The maturation promoting factor (MPF) is a key regulator of controlling G2/M phase transition in the meiotic maturation of oocyte and spermatocyte in animals, which is a complex of CDC2 (CDK1) and cyclin B. To better understand the molecular mechanism of oocyte and spermatocyte maturation in mud crab (Scylla paramamosain), the full length cDNA of cdc2 (Sp-cdc2) and cyclin B (Sp-cyclin B) were cloned and characterized. The full length cDNA of Sp-cdc2 gene is of 1593bp encoding a protein of 299 amino acids. Real-time quantitative PCR analysis revealed that the expression level of Sp-cdc2 in the ovary was higher than in other tissues (P0.05), meanwhile there was higher expression in T3 stage than in T1 and T2 stages (P
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2012.07.001