A novel thin-layer chromatography method to screen 1,3-propanediol producers

To date, there is no established protocol for the screening of 1,3-propanediol producers. The proposed method has a wide applicability to harness the commercial potential of microorganisms which produce 1,3-propanediol as the end product. Glycerol fermentation broth of 50 bacteria spotted on thin-la...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2012-11, Vol.39 (11), p.1713-1718
Main Authors: Anand, Pinki, Saxena, Rajendra Kumar
Format: Article
Language:English
Subjects:
Acids
Bacteria
Bacteria - isolation & purification
Bacteria - metabolism
Benzaldehydes
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Thin Layer - methods
Color
Coloring Agents - analysis
Culture Media
Ethanol
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Glycerol
Glycerol - metabolism
Hydrogen-Ion Concentration
Indicators and Reagents
Inorganic Chemistry
Ions
Life Sciences
Liquid chromatography
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Microorganisms
Propylene Glycols - analysis
Propylene Glycols - metabolism
Protocol
Reagents
Reproducibility of Results
Short Communication
Solvents
Solvents - chemistry
Studies
Thin layer chromatography
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Summary:To date, there is no established protocol for the screening of 1,3-propanediol producers. The proposed method has a wide applicability to harness the commercial potential of microorganisms which produce 1,3-propanediol as the end product. Glycerol fermentation broth of 50 bacteria spotted on thin-layer chromatography plates and run by appropriate solvent systems followed by colour development using vanillin reagent gave different coloured spots with most of the compounds present in the fermentation broth. The appearance of a purple-coloured spot of 1,3-propanediol with a retention factor ( R f ) of 0.62 forms the basis for the selection of 1,3-propanediol producers. Apart from being a rapid detection system the proposed method is pH independent and its authenticity was reconfirmed by HPLC.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-012-1161-6