Inter-individual Variability in OCT1 Expression and Its Relationship with OCT1 Genotype in Liver Samples from a Korean Population

To clarify inter-individual variation in the expression of organic cation transporter 1 (OCT1), the levels of OCT1 mRNA and protein from 65 human liver samples were examined by real-time PCR and Western blot analysis and were associated with OCT1 genotypes. The expression levels of OCT1 mRNA and pro...

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Published in:Drug metabolism and pharmacokinetics 2012, Vol.27 (5), p.530-535
Main Authors: Kim, Min-Hye, Shin, Ho Jung, Lim, Su Jeong, Park, Jung-Soon, Lee, Sang-Seop, Song, Im-Sook, Shin, Jae-Gook
Format: Article
Language:English
Subjects:
Asian Continental Ancestry Group - genetics
expression level
Female
Genotype
human liver
Humans
Individuality
inter-individual variations
Liver - metabolism
Liver - physiology
Male
Middle Aged
organic cation transporter 1 (OCT1)
Organic Cation Transporter 1 - biosynthesis
Organic Cation Transporter 1 - genetics
Organic Cation Transporter 1 - metabolism
Polymorphism, Single Nucleotide
Republic of Korea
RNA, Messenger - genetics
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Summary:To clarify inter-individual variation in the expression of organic cation transporter 1 (OCT1), the levels of OCT1 mRNA and protein from 65 human liver samples were examined by real-time PCR and Western blot analysis and were associated with OCT1 genotypes. The expression levels of OCT1 mRNA and protein in 65 liver samples of Korean origin were not normally distributed and varied by 23.6- and 15.9fold, respectively. OCT1 mRNA expression was correlated with OCT1 protein expression with a correlation coefficient of 0.641 (p < 0.0001). However, non-genetic factors, such as age, gender, and cholestasis, were not significantly associated with OCT1 expression. When quantitative expression levels were compared in relation to OCT1 promoter SNPs, there was no significant difference in OCT1 expression levels among the –1795 GG, GA, and AA genotypes. Moreover, expression levels of OCT1 were not changed in relation to the –1756 genotypes. Inter-individual variation in OCT1 mRNA and protein expression levels in the liver did not correlate with OCT1 genotypes or non-genetic factors, such as age, gender, and cholestasis. These results suggest that genetic and non-genetic factors may not be a significant contributing factor of variations in OCT1 expression from liver samples of Korean origin.
ISSN:1347-4367
1880-0920
DOI:10.2133/dmpk.DMPK-11-RG-109