Associations between polymorphisms in IL-12A, IL-12B, IL-12Rβ1, IL-27 gene and serum levels of IL-12p40, IL-27p28 with esophageal cancer

• Published in: Journal of cancer research and clinical oncology 2012-11, Vol.138 (11), p.1891-1900
• Main Authors: Tao, Yi-Peng, Wang, Wan-Ling, Li, Song-Yue, Zhang, Jian, Shi, Qi-Zhong, Zhao, Fen, Zhao, Bao-Sheng
• Format: Article
• Language: English
• Subjects: Aged; Alleles; Antineoplastic agents; Biological and medical sciences; Cancer Research; Case-Control Studies; Enzyme-Linked Immunosorbent Assay; Esophageal Neoplasms - blood; Esophageal Neoplasms - genetics; Esophagus; Female; Gastroenterology. Liver. Pancreas. Abdomen; Gene Frequency; Genetic Predisposition to Disease; Genotype; Hematology; Humans; Interleukin-12 - genetics; Interleukin-12 Subunit p40 - blood; Interleukin-12 Subunit p40 - genetics; Interleukins - genetics; Internal Medicine; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Paper; Pharmacology. Drug treatments; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide; Protein Subunits - blood; Receptors, Interleukin-12 - genetics; Tumors
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-012-1269-0

Purpose The aim of this study was to investigate whether IL-12A, IL-12B, IL-12Rβ1, and IL-27 gene polymorphisms and serum levels of IL-12, IL-27 are associated with esophageal cancer. Methods We genotyped IL-12A gene rs568408, IL-12B gene rs3212227, IL-12Rβ1 gene 378 C/G, IL-27 gene rs153109, rs17855750, and rs181206 polymorphisms in a case–control study of 426 esophageal cancer patients and 432 health controls, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and serum IL-12p40 and IL-27p28 levels were measured by enzyme-linked immunosorbent assay. Results Both serum IL-12p40 and IL-27p28 levels were significantly higher in controls than those in patients ( P