The inhibitory effect of a polysaccharide from Codonopsis pilosula on tumor growth and metastasis in vitro

• Published in: International journal of biological macromolecules 2012-12, Vol.51 (5), p.788-793
• Main Authors: Xin, Tao, Zhang, Fubin, Jiang, Qiuying, Chen, Chunhong, Huang, Dayong, Li, Yanju, Shen, Weixi, Jin, Yinghua, Sui, Guangjie
• Format: Article
• Language: English
• Subjects: adhesion; animal ovaries; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antitumor; cell adhesion; Cell Adhesion - drug effects; cell growth; Cell Line, Tumor; Cell Movement - drug effects; Cell Proliferation - drug effects; Chemical Phenomena; Codonopsis - chemistry; Codonopsis pilosula; Collagen - metabolism; Drug Combinations; Female; Gene Expression Regulation, Neoplastic - drug effects; Humans; Hyaluronan Receptors - metabolism; Laminin - metabolism; Metastasis; neoplasm cells; Neoplasm Invasiveness; Neoplasm Metastasis; Ovarian cancer; ovarian neoplasms; Ovarian Neoplasms - pathology; Polysaccharide; polysaccharides; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Proteoglycans - metabolism; roots; tissue repair
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2012.07.019

► An acidic polysaccharide (CPPA) was purified from Codonopsis pilosula. ► CPPA had a potent anti-metastatic effect on HO-8910 cells in vitro. ► CPPA had a significant inhibitor effect on the proliferation of HO-8910 cells. ► The CD44 expression on the HO-8910 cells was attenuated by CPPA treatment. ► CPPA may be a candidate compound for the prevention of tumor metastasis. In this study, we prepared an acidic polysaccharide (CPPA) from the roots of Codonopsis pilosula. The effects of CPPA on tumor cell growth, invasion, and migration were examined in vitro. The CPPA not only induced a potent inhibitory effect on the invasion and migration potential of human epithelial ovarian cancer HO-8910 cells in vitro, evaluated by wound healing, transwell and cell adhesion assays, but also had an efficient anti-proliferation effect on tumor cells. Moreover, the CD44 expression on the HO-8910 cells was also attenuated by CPPA treatment. Therefore, our results indicate that CPPA may be a potential candidate compound for the prevention of tumor metastasis, presumably by inhibiting invasion, migration and adhesion of tumor cells, as well as the CD44 expression on the tumor cells.