In Vitro Investigation of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone for Glycemic Control

2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC), a compound isolated and purified from the dried flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae), was investigated for its glucose control benefits using in vitro methods. DMC showed strong noncompetitive (IC50 of 43...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-10, Vol.60 (42), p.10683-10688
Main Authors: Hu, Ying-Chun, Luo, Ying-Di, Li, Lin, Joshi, Manoj Kumar, Lu, Yan-Hua
Format: Article
Language:English
Subjects:
alpha-amylase
alpha-glucosidase
Biological and medical sciences
Blood Glucose - analysis
buds
Caco-2 Cells
Chalcones - pharmacology
fasting
flowers
Food industries
Fundamental and applied biological sciences. Psychology
glucose
glycemic control
Humans
hydrogen peroxide
hyperglycemia
In Vitro Techniques
inhibitory concentration 50
insulin secretion
Myrtaceae
viability
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Summary:2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC), a compound isolated and purified from the dried flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae), was investigated for its glucose control benefits using in vitro methods. DMC showed strong noncompetitive (IC50 of 43 μM) inhibition of pancreatic α-amylase; it was, however, ineffective against intestinal α-glucosidase. In addition, DMC exhibited remarkable glucose transport inhibition effects in both simulated fasting and fed states in Caco-2 cell monolayers (P < 0.05). Besides, exposure of MIN6 cells to 250 μM H2O2 for 1 h caused a significant viability loss and insulin secretion reduction. Pretreatment of MIN6 cells with DMC for 2 h protected against the H2O2-induced decrease in glucose-stimulated insulin secretion in a dose-dependent manner and also enhanced the impaired basal insulin secretion. Such effects highlight the therapeutic potential of DMC in the management of hyperglycemia.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf303078r