Diterpenoids Isolated from Sideritis Species Protect Astrocytes against Oxidative Stress via Nrf2

Andalusol (1), conchitriol (2), and lagascatriol (3) are diterpenoids produced by Sideritis species. Hydrogen peroxide-induced oxidative stress in U373-MG cells was used as an in vitro model to evaluate the cytoprotective potential, based on antioxidant properties, of these isolated compounds and to...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2012-10, Vol.75 (10), p.1750-1758
Main Authors: González-Burgos, Elena, Carretero, M. Emilia, Gómez-Serranillos, M. Pilar
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Astrocytes - drug effects
Cell Survival - drug effects
Chromatography, High Pressure Liquid
Diterpenes - chemistry
Diterpenes - isolation & purification
Diterpenes - pharmacology
Glutathione - analysis
Humans
Hydrogen Peroxide - pharmacology
Malondialdehyde - analysis
Molecular Structure
NF-E2-Related Factor 2 - metabolism
Oxidative Stress - drug effects
Reactive Oxygen Species - pharmacology
Sideritis - chemistry
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Summary:Andalusol (1), conchitriol (2), and lagascatriol (3) are diterpenoids produced by Sideritis species. Hydrogen peroxide-induced oxidative stress in U373-MG cells was used as an in vitro model to evaluate the cytoprotective potential, based on antioxidant properties, of these isolated compounds and to investigate the underlying mechanisms. Experimental results revealed that pretreatment with compounds 1 and 3 restored H2O2-induced oxidative changes by increasing cell viability, attenuating morphological changes, inhibiting intracellular ROS production and lipid peroxidation, and enhancing the antioxidant defense system. Furthermore, nuclear factor E2-related factor 2 (Nrf2) signaling was involved in the protective mechanisms of 1–3. The present findings suggest that two of the compounds studied (1 and 3) might play a preventive role in neurodegenerative diseases associated with oxidative stress.
ISSN:0163-3864
1520-6025
DOI:10.1021/np300418m