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Reversible Two-Photon Photoswitching and Two-Photon Imaging of Immunofunctionalized Nanoparticles Targeted to Cancer Cells

Both photoswitchable fluorescent nanoparticles and photoactivatable fluorescent proteins have been used for super-resolution far-field imaging on the nanometer scale, but the photoactivating wavelength for such photochemical events generally falls in the near-UV (NUV) region (

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Published in:	Journal of the American Chemical Society 2011-01, Vol.133 (2), p.365-372
Main Authors:	Zhu, Ming-Qiang, Zhang, Guo-Feng, Li, Chong, Aldred, Matthew P, Chang, Emmanuel, Drezek, Rebekah A, Li, Alexander D. Q
Format:	Article
Language:	English
Subjects:	Benzopyrans - chemistry Cell Line, Tumor Fluorescence Humans Indoles - chemistry Nanoparticles - chemistry Neoplasms - pathology Nitro Compounds - chemistry Photochemical Processes Photons
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container_title	Journal of the American Chemical Society
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creator	Zhu, Ming-Qiang Zhang, Guo-Feng Li, Chong Aldred, Matthew P Chang, Emmanuel Drezek, Rebekah A Li, Alexander D. Q
description	Both photoswitchable fluorescent nanoparticles and photoactivatable fluorescent proteins have been used for super-resolution far-field imaging on the nanometer scale, but the photoactivating wavelength for such photochemical events generally falls in the near-UV (NUV) region (
doi_str_mv	10.1021/ja106895k
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Herein, two-photon absorption not only caused spiropyran−merocyanine photoisomerization within the particles but also imparted red fluorescence. 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Thus, we have demonstrated that alternating 780 nm NIR two-photon and 488 nm single-photon excitations induces reversible on−off fluorescence switching of immunotargeted nanoparticles in the human breast cancer cell line SK-BR-3. Herein, two-photon absorption not only caused spiropyran−merocyanine photoisomerization within the particles but also imparted red fluorescence. 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subjects	Benzopyrans - chemistry Cell Line, Tumor Fluorescence Humans Indoles - chemistry Nanoparticles - chemistry Neoplasms - pathology Nitro Compounds - chemistry Photochemical Processes Photons
title	Reversible Two-Photon Photoswitching and Two-Photon Imaging of Immunofunctionalized Nanoparticles Targeted to Cancer Cells
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