Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats

• Published in: Molecular and cellular biochemistry 2012-12, Vol.371 (1-2), p.147-156
• Main Authors: Cardoso, Andréia Machado, Bagatini, Margarete Dulce, Martins, Caroline Curry, Abdalla, Fátima Hussein, Zanini, Daniela, Schmatz, Roberta, Gutierres, Jessié, Pimentel, Victor Camera, Thomé, Gustavo, Leal, Claudio Alberto Martins, Vieira, Juliano Marchi, Stefanello, Naiara, da Silva Fiorin, Fernando, Baldissareli, Jucimara, Royes, Luiz Fernando Freire, Klein, Adriane Bello, Morsch, Vera Maria, Schetinger, Maria Rosa Chitolina
• Format: Article
• Language: English
• Subjects: 5'-Nucleotidase - metabolism; Adenosine Deaminase - metabolism; Adenosine diphosphate; Adenosine Diphosphate - metabolism; Adenosine Triphosphate - metabolism; Animals; ATP; Biochemistry; Biomedical and Life Sciences; Blood platelets; Blood Platelets - metabolism; Body weight; Cardiology; Enzymes; Exercise; Hydrolysis; Hypertension; Hypertension - blood; Life Sciences; Male; Medical Biochemistry; NG-Nitroarginine Methyl Ester - pharmacology; Nitric Oxide - antagonists & inhibitors; Nitric Oxide - biosynthesis; Nucleotidases; Nucleotides; Oncology; Physical Conditioning, Animal; Physical training; Platelet Aggregation - drug effects; Platelet Count; Proteins; Rats; Rats, Wistar; Rodents; Swimming
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-012-1431-7

In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of l -NAME. The rats were divided into four groups: control ( n  = 10), exercise ( n  = 10), l -NAME ( n  = 10), and exercise l -NAME ( n  = 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal’s body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5′-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from l -NAME-treated rats when compared to other groups ( p