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Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats
In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of l -NAME. The rats were divided into four groups: control ( n = 10), exercise ( n = 10),...
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| Published in: | Molecular and cellular biochemistry 2012-12, Vol.371 (1-2), p.147-156 |
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| container_title | Molecular and cellular biochemistry |
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| creator | Cardoso, Andréia Machado Bagatini, Margarete Dulce Martins, Caroline Curry Abdalla, Fátima Hussein Zanini, Daniela Schmatz, Roberta Gutierres, Jessié Pimentel, Victor Camera Thomé, Gustavo Leal, Claudio Alberto Martins Vieira, Juliano Marchi Stefanello, Naiara da Silva Fiorin, Fernando Baldissareli, Jucimara Royes, Luiz Fernando Freire Klein, Adriane Bello Morsch, Vera Maria Schetinger, Maria Rosa Chitolina |
| description | In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of
l
-NAME. The rats were divided into four groups: control (
n
= 10), exercise (
n
= 10),
l
-NAME (
n
= 10), and exercise
l
-NAME (
n
= 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal’s body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5′-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from
l
-NAME-treated rats when compared to other groups (
p
|
| doi_str_mv | 10.1007/s11010-012-1431-7 |
| format | article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1124752408</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A364198249</galeid><sourcerecordid>A364198249</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-29b84deffdd5f34a02d90e958215141567f2b74f280f8d1043dfbcae87c3cf593</originalsourceid><addsrcrecordid>eNp1kU9vFSEUxYnR2Gf1A7gxk7hxM5ULzAOWTVOtSRM37aoLwmMuT5p5MALT2G8vL6_1X2xYkMDvnHvgEPIW6AlQKj8WAAq0p8B6EBx6-YysYJC8Fxr0c7KinNJegZRH5FUpt7TBFOAlOWJMwwBrtSI35z8wu1Cwq9mGGOK2mzPeYaylQ1dTHxc3YaphtAVLZ6eK2daQYulC7ObJVpywscl33-5nzBVjCXfYNai8Ji-8nQq-ediPyfWn86uzi_7y6-cvZ6eXvRNc157pjRIjej-Og-fCUjZqinpQrGUUMKylZxspPFPUqxGo4KPfOItKOu78oPkx-XDwnXP6vmCpZheKw2myEdNSDAATcmCCqoa-_we9TUuOLV2jYBjWWnH4TW3thCZEn9rnuL2pOeVrAVoxsR978h-qrRF3waWIPrTzvwRwELicSsnozZzDzuZ7A9TsCzWHQk0r1OwLNbJp3j0EXjY7HH8pHhtsADsApV3FLeY_XvSk60_bp6pK</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1115569831</pqid></control><display><type>article</type><title>Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats</title><source>Springer Nature</source><creator>Cardoso, Andréia Machado ; Bagatini, Margarete Dulce ; Martins, Caroline Curry ; Abdalla, Fátima Hussein ; Zanini, Daniela ; Schmatz, Roberta ; Gutierres, Jessié ; Pimentel, Victor Camera ; Thomé, Gustavo ; Leal, Claudio Alberto Martins ; Vieira, Juliano Marchi ; Stefanello, Naiara ; da Silva Fiorin, Fernando ; Baldissareli, Jucimara ; Royes, Luiz Fernando Freire ; Klein, Adriane Bello ; Morsch, Vera Maria ; Schetinger, Maria Rosa Chitolina</creator><creatorcontrib>Cardoso, Andréia Machado ; Bagatini, Margarete Dulce ; Martins, Caroline Curry ; Abdalla, Fátima Hussein ; Zanini, Daniela ; Schmatz, Roberta ; Gutierres, Jessié ; Pimentel, Victor Camera ; Thomé, Gustavo ; Leal, Claudio Alberto Martins ; Vieira, Juliano Marchi ; Stefanello, Naiara ; da Silva Fiorin, Fernando ; Baldissareli, Jucimara ; Royes, Luiz Fernando Freire ; Klein, Adriane Bello ; Morsch, Vera Maria ; Schetinger, Maria Rosa Chitolina</creatorcontrib><description>In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of
l
-NAME. The rats were divided into four groups: control (
n
= 10), exercise (
n
= 10),
l
-NAME (
n
= 10), and exercise
l
-NAME (
n
= 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal’s body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5′-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from
l
-NAME-treated rats when compared to other groups (
p
< 0.05). A significant augment on platelet aggregation in
l
-NAME group was also observed. Exercise training was efficient in preventing these alterations in the exercise
l
-NAME group, besides showing a significant hypotensive effect. In conclusion, our results clearly indicated a protector action of moderate intensity exercise on nucleotides and nucleoside hydrolysis and on platelet aggregation, which highlights the exercise training effect to avoid hypertension complications related to ecto-nucleotidase activities.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-012-1431-7</identifier><identifier>PMID: 22915168</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>5'-Nucleotidase - metabolism ; Adenosine Deaminase - metabolism ; Adenosine diphosphate ; Adenosine Diphosphate - metabolism ; Adenosine Triphosphate - metabolism ; Animals ; ATP ; Biochemistry ; Biomedical and Life Sciences ; Blood platelets ; Blood Platelets - metabolism ; Body weight ; Cardiology ; Enzymes ; Exercise ; Hydrolysis ; Hypertension ; Hypertension - blood ; Life Sciences ; Male ; Medical Biochemistry ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - biosynthesis ; Nucleotidases ; Nucleotides ; Oncology ; Physical Conditioning, Animal ; Physical training ; Platelet Aggregation - drug effects ; Platelet Count ; Proteins ; Rats ; Rats, Wistar ; Rodents ; Swimming</subject><ispartof>Molecular and cellular biochemistry, 2012-12, Vol.371 (1-2), p.147-156</ispartof><rights>Springer Science+Business Media, LLC. 2012</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer Science+Business Media New York 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-29b84deffdd5f34a02d90e958215141567f2b74f280f8d1043dfbcae87c3cf593</citedby><cites>FETCH-LOGICAL-c439t-29b84deffdd5f34a02d90e958215141567f2b74f280f8d1043dfbcae87c3cf593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22915168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cardoso, Andréia Machado</creatorcontrib><creatorcontrib>Bagatini, Margarete Dulce</creatorcontrib><creatorcontrib>Martins, Caroline Curry</creatorcontrib><creatorcontrib>Abdalla, Fátima Hussein</creatorcontrib><creatorcontrib>Zanini, Daniela</creatorcontrib><creatorcontrib>Schmatz, Roberta</creatorcontrib><creatorcontrib>Gutierres, Jessié</creatorcontrib><creatorcontrib>Pimentel, Victor Camera</creatorcontrib><creatorcontrib>Thomé, Gustavo</creatorcontrib><creatorcontrib>Leal, Claudio Alberto Martins</creatorcontrib><creatorcontrib>Vieira, Juliano Marchi</creatorcontrib><creatorcontrib>Stefanello, Naiara</creatorcontrib><creatorcontrib>da Silva Fiorin, Fernando</creatorcontrib><creatorcontrib>Baldissareli, Jucimara</creatorcontrib><creatorcontrib>Royes, Luiz Fernando Freire</creatorcontrib><creatorcontrib>Klein, Adriane Bello</creatorcontrib><creatorcontrib>Morsch, Vera Maria</creatorcontrib><creatorcontrib>Schetinger, Maria Rosa Chitolina</creatorcontrib><title>Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of
l
-NAME. The rats were divided into four groups: control (
n
= 10), exercise (
n
= 10),
l
-NAME (
n
= 10), and exercise
l
-NAME (
n
= 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal’s body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5′-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from
l
-NAME-treated rats when compared to other groups (
p
< 0.05). A significant augment on platelet aggregation in
l
-NAME group was also observed. Exercise training was efficient in preventing these alterations in the exercise
l
-NAME group, besides showing a significant hypotensive effect. In conclusion, our results clearly indicated a protector action of moderate intensity exercise on nucleotides and nucleoside hydrolysis and on platelet aggregation, which highlights the exercise training effect to avoid hypertension complications related to ecto-nucleotidase activities.</description><subject>5'-Nucleotidase - metabolism</subject><subject>Adenosine Deaminase - metabolism</subject><subject>Adenosine diphosphate</subject><subject>Adenosine Diphosphate - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>ATP</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Blood platelets</subject><subject>Blood Platelets - metabolism</subject><subject>Body weight</subject><subject>Cardiology</subject><subject>Enzymes</subject><subject>Exercise</subject><subject>Hydrolysis</subject><subject>Hypertension</subject><subject>Hypertension - blood</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical Biochemistry</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nucleotidases</subject><subject>Nucleotides</subject><subject>Oncology</subject><subject>Physical Conditioning, Animal</subject><subject>Physical training</subject><subject>Platelet Aggregation - drug effects</subject><subject>Platelet Count</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Swimming</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kU9vFSEUxYnR2Gf1A7gxk7hxM5ULzAOWTVOtSRM37aoLwmMuT5p5MALT2G8vL6_1X2xYkMDvnHvgEPIW6AlQKj8WAAq0p8B6EBx6-YysYJC8Fxr0c7KinNJegZRH5FUpt7TBFOAlOWJMwwBrtSI35z8wu1Cwq9mGGOK2mzPeYaylQ1dTHxc3YaphtAVLZ6eK2daQYulC7ObJVpywscl33-5nzBVjCXfYNai8Ji-8nQq-ediPyfWn86uzi_7y6-cvZ6eXvRNc157pjRIjej-Og-fCUjZqinpQrGUUMKylZxspPFPUqxGo4KPfOItKOu78oPkx-XDwnXP6vmCpZheKw2myEdNSDAATcmCCqoa-_we9TUuOLV2jYBjWWnH4TW3thCZEn9rnuL2pOeVrAVoxsR978h-qrRF3waWIPrTzvwRwELicSsnozZzDzuZ7A9TsCzWHQk0r1OwLNbJp3j0EXjY7HH8pHhtsADsApV3FLeY_XvSk60_bp6pK</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Cardoso, Andréia Machado</creator><creator>Bagatini, Margarete Dulce</creator><creator>Martins, Caroline Curry</creator><creator>Abdalla, Fátima 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training prevents ecto-nucleotidases alterations in platelets of hypertensive rats</title><author>Cardoso, Andréia Machado ; Bagatini, Margarete Dulce ; Martins, Caroline Curry ; Abdalla, Fátima Hussein ; Zanini, Daniela ; Schmatz, Roberta ; Gutierres, Jessié ; Pimentel, Victor Camera ; Thomé, Gustavo ; Leal, Claudio Alberto Martins ; Vieira, Juliano Marchi ; Stefanello, Naiara ; da Silva Fiorin, Fernando ; Baldissareli, Jucimara ; Royes, Luiz Fernando Freire ; Klein, Adriane Bello ; Morsch, Vera Maria ; Schetinger, Maria Rosa Chitolina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-29b84deffdd5f34a02d90e958215141567f2b74f280f8d1043dfbcae87c3cf593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>5'-Nucleotidase - metabolism</topic><topic>Adenosine Deaminase - metabolism</topic><topic>Adenosine diphosphate</topic><topic>Adenosine Diphosphate - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>ATP</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Blood platelets</topic><topic>Blood Platelets - metabolism</topic><topic>Body weight</topic><topic>Cardiology</topic><topic>Enzymes</topic><topic>Exercise</topic><topic>Hydrolysis</topic><topic>Hypertension</topic><topic>Hypertension - blood</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical Biochemistry</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nucleotidases</topic><topic>Nucleotides</topic><topic>Oncology</topic><topic>Physical Conditioning, Animal</topic><topic>Physical training</topic><topic>Platelet Aggregation - drug effects</topic><topic>Platelet Count</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Swimming</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardoso, Andréia Machado</creatorcontrib><creatorcontrib>Bagatini, Margarete Dulce</creatorcontrib><creatorcontrib>Martins, Caroline Curry</creatorcontrib><creatorcontrib>Abdalla, Fátima Hussein</creatorcontrib><creatorcontrib>Zanini, Daniela</creatorcontrib><creatorcontrib>Schmatz, Roberta</creatorcontrib><creatorcontrib>Gutierres, Jessié</creatorcontrib><creatorcontrib>Pimentel, Victor Camera</creatorcontrib><creatorcontrib>Thomé, Gustavo</creatorcontrib><creatorcontrib>Leal, Claudio Alberto Martins</creatorcontrib><creatorcontrib>Vieira, Juliano Marchi</creatorcontrib><creatorcontrib>Stefanello, Naiara</creatorcontrib><creatorcontrib>da Silva Fiorin, Fernando</creatorcontrib><creatorcontrib>Baldissareli, Jucimara</creatorcontrib><creatorcontrib>Royes, Luiz Fernando Freire</creatorcontrib><creatorcontrib>Klein, Adriane 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Chitolina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats</atitle><jtitle>Molecular and cellular biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2012-12-01</date><risdate>2012</risdate><volume>371</volume><issue>1-2</issue><spage>147</spage><epage>156</epage><pages>147-156</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of
l
-NAME. The rats were divided into four groups: control (
n
= 10), exercise (
n
= 10),
l
-NAME (
n
= 10), and exercise
l
-NAME (
n
= 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal’s body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5′-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from
l
-NAME-treated rats when compared to other groups (
p
< 0.05). A significant augment on platelet aggregation in
l
-NAME group was also observed. Exercise training was efficient in preventing these alterations in the exercise
l
-NAME group, besides showing a significant hypotensive effect. In conclusion, our results clearly indicated a protector action of moderate intensity exercise on nucleotides and nucleoside hydrolysis and on platelet aggregation, which highlights the exercise training effect to avoid hypertension complications related to ecto-nucleotidase activities.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>22915168</pmid><doi>10.1007/s11010-012-1431-7</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 2012-12, Vol.371 (1-2), p.147-156 |
| issn | 0300-8177 1573-4919 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1124752408 |
| source | Springer Nature |
| subjects | 5'-Nucleotidase - metabolism Adenosine Deaminase - metabolism Adenosine diphosphate Adenosine Diphosphate - metabolism Adenosine Triphosphate - metabolism Animals ATP Biochemistry Biomedical and Life Sciences Blood platelets Blood Platelets - metabolism Body weight Cardiology Enzymes Exercise Hydrolysis Hypertension Hypertension - blood Life Sciences Male Medical Biochemistry NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide - antagonists & inhibitors Nitric Oxide - biosynthesis Nucleotidases Nucleotides Oncology Physical Conditioning, Animal Physical training Platelet Aggregation - drug effects Platelet Count Proteins Rats Rats, Wistar Rodents Swimming |
| title | Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T05%3A16%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exercise%20training%20prevents%20ecto-nucleotidases%20alterations%20in%20platelets%20of%20hypertensive%20rats&rft.jtitle=Molecular%20and%20cellular%20biochemistry&rft.au=Cardoso,%20Andr%C3%A9ia%20Machado&rft.date=2012-12-01&rft.volume=371&rft.issue=1-2&rft.spage=147&rft.epage=156&rft.pages=147-156&rft.issn=0300-8177&rft.eissn=1573-4919&rft_id=info:doi/10.1007/s11010-012-1431-7&rft_dat=%3Cgale_proqu%3EA364198249%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c439t-29b84deffdd5f34a02d90e958215141567f2b74f280f8d1043dfbcae87c3cf593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1115569831&rft_id=info:pmid/22915168&rft_galeid=A364198249&rfr_iscdi=true |