Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases

• Published in: Journal of digestive diseases 2012-11, Vol.13 (11), p.571-578
• Main Authors: Ghanem, Lobna Y, Nosseir, Mona MF, Lotfi, Abeya A, Mohamed, Ayat S, Ibrahim, Refaat A, Hassanein, Moutaz H, Mansour, Eman, Makhlouf, Manal M, Fouad, Yasser M, El-Khayat, Hisham R
• Format: Article
• Language: English
• Subjects: AC133 Antigen; Adult; Antigens, CD - metabolism; Antigens, CD34 - metabolism; Biopsy; Cell Movement - physiology; Chronic Disease; Female; Flow Cytometry; Glycoproteins - metabolism; Hematopoietic Stem Cell Mobilization; hematopoietic stem cells; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - metabolism; hepatic progenitors; Humans; liver diseases; Liver Diseases - pathology; Liver Diseases - physiopathology; Liver Regeneration - physiology; Male; Middle Aged; oval cells; Peptides - metabolism; Severity of Illness Index
• ISSN: 1751-2972; 1751-2980
• DOI: 10.1111/j.1751-2980.2012.00634.x

Objective The present study was aimed to investigate and compare the kinetics of bone marrow‐derived hematopoietic stem cells (BMHSC) migration in the peripheral blood and liver in response to liver injury in patients with chronic liver disease (CLD). Methods In all, 45 CLD patients staged with Child–Pugh A, B and C and 15 healthy participants were evaluated for the concentration of circulating BMHSC by a flow cytometric analysis of CD133+/CD34+ cells. In addition, homing BMHSC and hepatic progenitors were assessed by the immunohistochemical detection of CD133+ and OV6+ cells in liver biopsy specimens from Child–Pugh A and B patients. Results No significant difference in the percentage of circulating CD133+/CD34+ cells was observed among all groups of patients. In liver tissues, OV6+ cells increased significantly in Child–Pugh B cases (P