Contamination control of microbe Ziziphus spina [christti] seed in vitro culture

Ziziphus spina [christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed g...

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Bibliographic Details
Published in:Trees (Berlin, West) West), 2012-08, Vol.26 (4), p.1299-1304
Main Authors: Ahmadi, Elahe, Nasr, Seyed Mohammad Hosseini, Jalilvand, Hamid, Savadkoohi, Sekineh Kiani
Format: Article
Language:English
Subjects:
Agriculture
Alternaria
Aspergillus
Benomyl
Biomedical and Life Sciences
Chemical treatment
Contaminants
Drought
Economic importance
Forestry
Fungicides
Fusarium
Hydrogen peroxide
Life Sciences
Microbial contamination
Microorganisms
Original Paper
Penicillium
Plant Anatomy/Development
Plant Pathology
Plant Physiology
Plant Sciences
Pollution control
Rhizopus
Seed germination
Seedlings
Seeds
Sterilization
Xanthomonas
Ziziphus
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Summary:Ziziphus spina [christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in Z. spina [christti] seeds and to enhance the seed germination. Different chemical treatments were used in surface sterilization of seeds: (Treatment 1) sodium hypochlorite (NaOCl) in concentrations of 1, 2 and 4% for 20 min. (Treatment 2) hydrogen peroxide (H 2 O 2 ) in concentration of 4, 8, 12% treated for 10 min. (Treatment 3) 1% mercuric chloride (HgCl 2 ) at duration 10, 15, 20, 25 min. Seeds were scarified and aseptically, planted on agar Murashige and Skoog (MS) medium. Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included Xanthomonas sp. While Fungal isolates were Fusarium, Penicillium, Alternaria, Rhizopus, and Aspergillus . Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for Z. spina [christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment.
ISSN:0931-1890
1432-2285
DOI:10.1007/s00468-012-0705-8