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Contamination control of microbe Ziziphus spina [christti] seed in vitro culture
Ziziphus spina [christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed g...
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| Published in: | Trees (Berlin, West) West), 2012-08, Vol.26 (4), p.1299-1304 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c458t-f278269c911f7fcd23d87724ac8076f8776bd2c5142868210fa3f44b4c2c7e1a3 |
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| cites | cdi_FETCH-LOGICAL-c458t-f278269c911f7fcd23d87724ac8076f8776bd2c5142868210fa3f44b4c2c7e1a3 |
| container_end_page | 1304 |
| container_issue | 4 |
| container_start_page | 1299 |
| container_title | Trees (Berlin, West) |
| container_volume | 26 |
| creator | Ahmadi, Elahe Nasr, Seyed Mohammad Hosseini Jalilvand, Hamid Savadkoohi, Sekineh Kiani |
| description | Ziziphus spina
[christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in
Z. spina
[christti] seeds and to enhance the seed germination. Different chemical treatments were used in surface sterilization of seeds: (Treatment 1) sodium hypochlorite (NaOCl) in concentrations of 1, 2 and 4% for 20 min. (Treatment 2) hydrogen peroxide (H
2
O
2
) in concentration of 4, 8, 12% treated for 10 min. (Treatment 3) 1% mercuric chloride (HgCl
2
) at duration 10, 15, 20, 25 min. Seeds were scarified and aseptically, planted on agar Murashige and Skoog (MS) medium. Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included
Xanthomonas
sp. While Fungal isolates were
Fusarium, Penicillium, Alternaria, Rhizopus,
and
Aspergillus
. Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for
Z. spina
[christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment. |
| doi_str_mv | 10.1007/s00468-012-0705-8 |
| format | article |
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[christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in
Z. spina
[christti] seeds and to enhance the seed germination. Different chemical treatments were used in surface sterilization of seeds: (Treatment 1) sodium hypochlorite (NaOCl) in concentrations of 1, 2 and 4% for 20 min. (Treatment 2) hydrogen peroxide (H
2
O
2
) in concentration of 4, 8, 12% treated for 10 min. (Treatment 3) 1% mercuric chloride (HgCl
2
) at duration 10, 15, 20, 25 min. Seeds were scarified and aseptically, planted on agar Murashige and Skoog (MS) medium. Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included
Xanthomonas
sp. While Fungal isolates were
Fusarium, Penicillium, Alternaria, Rhizopus,
and
Aspergillus
. Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for
Z. spina
[christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment.</description><identifier>ISSN: 0931-1890</identifier><identifier>EISSN: 1432-2285</identifier><identifier>DOI: 10.1007/s00468-012-0705-8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Agriculture ; Alternaria ; Aspergillus ; Benomyl ; Biomedical and Life Sciences ; Chemical treatment ; Contaminants ; Drought ; Economic importance ; Forestry ; Fungicides ; Fusarium ; Hydrogen peroxide ; Life Sciences ; Microbial contamination ; Microorganisms ; Original Paper ; Penicillium ; Plant Anatomy/Development ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Pollution control ; Rhizopus ; Seed germination ; Seedlings ; Seeds ; Sterilization ; Xanthomonas ; Ziziphus</subject><ispartof>Trees (Berlin, West), 2012-08, Vol.26 (4), p.1299-1304</ispartof><rights>The Author(s) 2012</rights><rights>Springer-Verlag 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-f278269c911f7fcd23d87724ac8076f8776bd2c5142868210fa3f44b4c2c7e1a3</citedby><cites>FETCH-LOGICAL-c458t-f278269c911f7fcd23d87724ac8076f8776bd2c5142868210fa3f44b4c2c7e1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27900,27901</link.rule.ids></links><search><creatorcontrib>Ahmadi, Elahe</creatorcontrib><creatorcontrib>Nasr, Seyed Mohammad Hosseini</creatorcontrib><creatorcontrib>Jalilvand, Hamid</creatorcontrib><creatorcontrib>Savadkoohi, Sekineh Kiani</creatorcontrib><title>Contamination control of microbe Ziziphus spina [christti] seed in vitro culture</title><title>Trees (Berlin, West)</title><addtitle>Trees</addtitle><description>Ziziphus spina
[christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in
Z. spina
[christti] seeds and to enhance the seed germination. Different chemical treatments were used in surface sterilization of seeds: (Treatment 1) sodium hypochlorite (NaOCl) in concentrations of 1, 2 and 4% for 20 min. (Treatment 2) hydrogen peroxide (H
2
O
2
) in concentration of 4, 8, 12% treated for 10 min. (Treatment 3) 1% mercuric chloride (HgCl
2
) at duration 10, 15, 20, 25 min. Seeds were scarified and aseptically, planted on agar Murashige and Skoog (MS) medium. Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included
Xanthomonas
sp. While Fungal isolates were
Fusarium, Penicillium, Alternaria, Rhizopus,
and
Aspergillus
. Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for
Z. spina
[christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment.</description><subject>Agriculture</subject><subject>Alternaria</subject><subject>Aspergillus</subject><subject>Benomyl</subject><subject>Biomedical and Life Sciences</subject><subject>Chemical treatment</subject><subject>Contaminants</subject><subject>Drought</subject><subject>Economic importance</subject><subject>Forestry</subject><subject>Fungicides</subject><subject>Fusarium</subject><subject>Hydrogen peroxide</subject><subject>Life Sciences</subject><subject>Microbial contamination</subject><subject>Microorganisms</subject><subject>Original Paper</subject><subject>Penicillium</subject><subject>Plant Anatomy/Development</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Pollution control</subject><subject>Rhizopus</subject><subject>Seed germination</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Sterilization</subject><subject>Xanthomonas</subject><subject>Ziziphus</subject><issn>0931-1890</issn><issn>1432-2285</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LxDAQxYMouK5-AG8BL16imWnapEdZ_AeCHvSiSOimiZul29akFfTTm6UeRPA0M_B7b2YeIcfAz4BzeR45F4ViHJBxyXOmdsgMRIYMUeW7ZMbLDBioku-TgxjXnPOsAJyRh0XXDtXGt9Xgu5aaNIWuoZ2jG29Ct7T02X_5fjVGGvtE0RezCj4Og3-l0dqa-pZ--KShZmyGMdhDsueqJtqjnzonT1eXj4sbdnd_fbu4uGNG5GpgDqXCojQlgJPO1JjVSkoUlVFcFi71xbJGk4NAVSgE7qrMCbEUBo20UGVzcjr59qF7H20c9MZHY5umam03Rg2AOaLI8iKhJ3_QdTeGNl2ngWMhIW3cUjBR6e0Yg3W6D35Thc8E6W3GespYp4z1NmOtkgYnTUxs-2bDb-f_RN-9h34Q</recordid><startdate>20120801</startdate><enddate>20120801</enddate><creator>Ahmadi, Elahe</creator><creator>Nasr, Seyed Mohammad Hosseini</creator><creator>Jalilvand, Hamid</creator><creator>Savadkoohi, Sekineh Kiani</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>SOI</scope><scope>7QL</scope><scope>7TV</scope><scope>7U6</scope></search><sort><creationdate>20120801</creationdate><title>Contamination control of microbe Ziziphus spina [christti] seed in vitro culture</title><author>Ahmadi, Elahe ; Nasr, Seyed Mohammad Hosseini ; Jalilvand, Hamid ; Savadkoohi, Sekineh Kiani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-f278269c911f7fcd23d87724ac8076f8776bd2c5142868210fa3f44b4c2c7e1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Agriculture</topic><topic>Alternaria</topic><topic>Aspergillus</topic><topic>Benomyl</topic><topic>Biomedical and Life Sciences</topic><topic>Chemical treatment</topic><topic>Contaminants</topic><topic>Drought</topic><topic>Economic importance</topic><topic>Forestry</topic><topic>Fungicides</topic><topic>Fusarium</topic><topic>Hydrogen peroxide</topic><topic>Life Sciences</topic><topic>Microbial contamination</topic><topic>Microorganisms</topic><topic>Original Paper</topic><topic>Penicillium</topic><topic>Plant Anatomy/Development</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Pollution control</topic><topic>Rhizopus</topic><topic>Seed germination</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Sterilization</topic><topic>Xanthomonas</topic><topic>Ziziphus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahmadi, Elahe</creatorcontrib><creatorcontrib>Nasr, Seyed Mohammad Hosseini</creatorcontrib><creatorcontrib>Jalilvand, Hamid</creatorcontrib><creatorcontrib>Savadkoohi, Sekineh Kiani</creatorcontrib><collection>SpringerOpen</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environment Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Pollution Abstracts</collection><collection>Sustainability Science Abstracts</collection><jtitle>Trees (Berlin, West)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahmadi, Elahe</au><au>Nasr, Seyed Mohammad Hosseini</au><au>Jalilvand, Hamid</au><au>Savadkoohi, Sekineh Kiani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Contamination control of microbe Ziziphus spina [christti] seed in vitro culture</atitle><jtitle>Trees (Berlin, West)</jtitle><stitle>Trees</stitle><date>2012-08-01</date><risdate>2012</risdate><volume>26</volume><issue>4</issue><spage>1299</spage><epage>1304</epage><pages>1299-1304</pages><issn>0931-1890</issn><eissn>1432-2285</eissn><abstract>Ziziphus spina
[christti] is a naturally distributed tree in subtropical, arid and semi-arid parts of Iran. It is ecologically and economically important due to its tolerance to drought and salinity. Most tree seeds are infected with parasitic and saprophytic microorganisms which decrease the seed germination and seedling establishment. The goal of this paper was to evaluate the ability of selected chemical solutions to inhibit the growth of variety of microbial contaminants in
Z. spina
[christti] seeds and to enhance the seed germination. Different chemical treatments were used in surface sterilization of seeds: (Treatment 1) sodium hypochlorite (NaOCl) in concentrations of 1, 2 and 4% for 20 min. (Treatment 2) hydrogen peroxide (H
2
O
2
) in concentration of 4, 8, 12% treated for 10 min. (Treatment 3) 1% mercuric chloride (HgCl
2
) at duration 10, 15, 20, 25 min. Seeds were scarified and aseptically, planted on agar Murashige and Skoog (MS) medium. Contaminants were identified according to their morphological and cultural characteristics. Bacterial contaminants included
Xanthomonas
sp. While Fungal isolates were
Fusarium, Penicillium, Alternaria, Rhizopus,
and
Aspergillus
. Our experiment reveals that 4% NaOCl followed by benomyl is the best sterilization treatment for
Z. spina
[christti] seeds, since the highest number of germination and highest number of sterilized seeds was observed after this treatment.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s00468-012-0705-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0931-1890 |
| ispartof | Trees (Berlin, West), 2012-08, Vol.26 (4), p.1299-1304 |
| issn | 0931-1890 1432-2285 |
| language | eng |
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| source | Springer Link |
| subjects | Agriculture Alternaria Aspergillus Benomyl Biomedical and Life Sciences Chemical treatment Contaminants Drought Economic importance Forestry Fungicides Fusarium Hydrogen peroxide Life Sciences Microbial contamination Microorganisms Original Paper Penicillium Plant Anatomy/Development Plant Pathology Plant Physiology Plant Sciences Pollution control Rhizopus Seed germination Seedlings Seeds Sterilization Xanthomonas Ziziphus |
| title | Contamination control of microbe Ziziphus spina [christti] seed in vitro culture |
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