Pectobacterium spp. Associated with Bacterial Stem Rot Syndrome of Potato in Canada

Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the...

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Bibliographic Details
Published in:Phytopathology 2012-10, Vol.102 (10), p.937-947
Main Authors: DE BOER, S. H, LI, X, WARD, L. J
Format: Article
Language:English
Subjects:
Bacteria
Base Sequence
Biological and medical sciences
Canada
DNA Primers
Fundamental and applied biological sciences. Psychology
Pectobacterium
Pectobacterium - classification
Pectobacterium - pathogenicity
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
Plant Diseases - microbiology
Polymerase Chain Reaction
Solanum tuberosum
Solanum tuberosum - microbiology
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Summary:Pectobacterium atrosepticum, P. carotovorum subsp. brasiliensis, P. carotovorum subsp. carotovorum, and P. wasabiae were detected in potato stems with blackleg symptoms using species- and subspecies-specific polymerase chain reaction (PCR). The tests included a new assay for P. wasabiae based on the phytase gene sequence. Identification of isolates from diseased stems by biochemical or physiological characterization, PCR, and multi-locus sequence typing (MLST) largely confirmed the PCR detection of Pectobacterium spp. in stem samples. P. atrosepticum was most commonly present but was the sole Pectobacterium sp. detected in only 52% of the diseased stems. P. wasabiae was most frequently present in combination with P. atrosepticum and was the sole Pectobacterium sp. detected in 13% of diseased stems. Pathogenicity of P. wasabiae on potato and its capacity to cause blackleg disease were demonstrated by stem inoculation and its isolation as the sole Pectobacterium sp. from field-grown diseased plants produced from inoculated seed tubers. Incidence of P. carotovorum subsp. brasiliensis was low in diseased stems, and the ability of Canadian strains to cause blackleg in plants grown from inoculated tubers was not confirmed. Canadian isolates of P. carotovorum subsp. brasiliensis differed from Brazilian isolates in diagnostic biochemical tests but conformed to the subspecies in PCR specificity and typing by MLST.
ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO-04-12-0083-R