ARID1A expression loss in gastric cancer: pathway-dependent roles with and without Epstein–Barr virus infection and microsatellite instability

• Published in: Virchows Archiv : an international journal of pathology 2012-10, Vol.461 (4), p.367-377
• Main Authors: Abe, Hiroyuki, Maeda, Daichi, Hino, Rumi, Otake, Yuya, Isogai, Maya, Ushiku, Aya Shinozaki, Matsusaka, Keisuke, Kunita, Akiko, Ushiku, Tetsuo, Uozaki, Hiroshi, Tateishi, Yoko, Hishima, Tsunekazu, Iwasaki, Yoshiaki, Ishikawa, Shumpei, Fukayama, Masashi
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - mortality; Aged; Biological and medical sciences; Carcinogenesis; Cell Line, Tumor; Chromatin remodeling; Disease Progression; DNA, Neoplasm - genetics; Down-Regulation - genetics; Down-Regulation - physiology; Epithelial cells; Epstein-Barr virus; Epstein-Barr Virus Infections - genetics; Epstein-Barr Virus Infections - physiopathology; Female; Gastric cancer; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression Regulation, Neoplastic - genetics; Gene Expression Regulation, Neoplastic - physiology; Histology; Humans; Immunohistochemistry; Infection; Infectious diseases; Investigative techniques, diagnostic techniques (general aspects); Lymph nodes; Lymphatic Metastasis - genetics; Lymphatic Metastasis - physiopathology; Lymphoma; Lymphoma - genetics; Lymphoma - metabolism; Male; Medical sciences; Medicine; Medicine & Public Health; Metastases; Microsatellite Instability; Microsatellites; Middle Aged; MLH1 protein; Mutation; MutL Protein Homolog 1; Nasopharyngeal carcinoma; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Original Article; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Prognosis; Retrospective Studies; Signal Transduction - genetics; Signal Transduction - physiology; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - mortality; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Sucrose; Survival Rate; Transcription Factors - genetics; Transcription Factors - metabolism; Tumor cell lines; Tumors; Viral diseases
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-012-1303-2

The AT-rich interactive domain 1A gene (ARID1A), which encodes one of the subunits in the Switch/Sucrose Nonfermentable chromatin remodeling complex, carries mutations and is responsible for loss of protein expression in gastric carcinoma, particularly with Epstein–Barr virus (EBV) infection and a microsatellite instability-high phenotype. We used immunohistochemistry to investigate the significance of ARID1A loss in 857 gastric carcinoma cases, including 67 EBV(+) and 136 MLH1-lost gastric carcinomas (corresponding to a microsatellite instability-high phenotype). Loss of ARID1A expression was significantly more frequent in EBV(+) (23/67; 34 %) and MLH1-lost (40/136; 29 %) gastric carcinomas than in EBV(−)MLH1-preserved (32/657; 5 %) gastric carcinomas ( P