Dose-Dependent Influence of Sevoflurane Anesthesia on Neuronal Survival and Cognitive Outcome After Transient Forebrain Ischemia in Sprague-Dawley Rats

Background Volatile anesthetics reduce postischemic neurohistopathological injury and improve neurological outcome in various animal models. However, the isoflurane concentrations above 1 minimum alveolar concentration (MAC) have been associated with reduced neuronal survival and impaired functional...

Full description

Saved in:
Bibliographic Details
Published in:Neurocritical care 2011-12, Vol.15 (3), p.577-584
Main Authors: Lasarzik, Irina, Noppens, Rüdiger R., Wolf, Thorsten, Bauer, Henrike, Luh, Clara, Werner, Christian, Engelhard, Kristin, Thal, Serge C.
Format: Article
Language:English
Subjects:
Anesthesia
Anesthetics, Inhalation - pharmacology
Animal cognition
Animals
Blood pressure
Catheters
Cell Survival - drug effects
Critical Care Medicine
Discrimination (Psychology) - drug effects
Dose-Response Relationship, Drug
Exploratory Behavior - drug effects
Hippocampus - drug effects
Hippocampus - pathology
Indexing in process
Intensive
Internal Medicine
Ischemia
Ischemic Attack, Transient - pathology
Male
Medicine
Medicine & Public Health
Memory
Mental Recall - drug effects
Methyl Ethers - pharmacology
Neurologic Examination - drug effects
Neurology
Neurons - drug effects
Neurons - pathology
Normal distribution
Pattern Recognition, Visual - drug effects
Prosencephalon - blood supply
Prosencephalon - drug effects
Prosencephalon - pathology
Rats
Rats, Sprague-Dawley
Reaction Time - drug effects
Translational Research
Veins & arteries
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Volatile anesthetics reduce postischemic neurohistopathological injury and improve neurological outcome in various animal models. However, the isoflurane concentrations above 1 minimum alveolar concentration (MAC) have been associated with reduced neuronal survival and impaired functional outcome. The aim of this study was to evaluate if 1.8 MAC sevoflurane alters postischemic neuronal survival and neurologic outcome compared with 0.45 MAC sevoflurane. Methods In this study, 20 fasted male Sprague-Dawley rats were randomly assigned to treatment groups with 1 or 4 vol.% sevoflurane end-tidal concentration. Cerebral ischemia was induced by bilateral carotid artery occlusion and hemorrhagic hypotension (BCAO). The cognitive outcome was assessed after 7 days using the object recognition test. Animals were then re-anesthetized and brains were removed for neurohistopathological analysis of the hippocampus (CA1) and cortex using hematoxylin-eosin staining. Results Physiologic parameters were not different between both the treatment groups. The number of viable neurons (median [Q1, Q3]) in the CA1 region on postischemic day 7 was increased after high-dose sevoflurane compared with low-dose sevoflurane (1645 [453, 1825] vs. 3222 [2920, 3993] neurons/ROI, P  
ISSN:1541-6933
1556-0961
DOI:10.1007/s12028-011-9562-3