Cloning and Characterization of the Beer Foaming Gene CFG1 from Saccharomyces pastorianus

Foam production is an essential characteristic of beer, generated mainly from the proteins present in the malt and, to a minor extent, from the mannoproteins in brewer's yeast cell walls. Here, we describe the isolation and characterization of the novel fermentation gene CFG1 (Carlsbergensis fo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2012-10, Vol.60 (43), p.10796-10807
Main Authors: Blasco, Lucía, Veiga-Crespo, Patricia, Sánchez-Pérez, Angeles, Villa, Tomás G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Beer - analysis
Beer - microbiology
Beers
Biological and medical sciences
brewers yeast
cell walls
Cloning, Molecular
Fermented food industries
foaming
Food industries
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
genes
Molecular Sequence Data
Saccharomyces - chemistry
Saccharomyces - genetics
Saccharomyces - metabolism
Saccharomyces cerevisiae
Sequence Alignment
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Foam production is an essential characteristic of beer, generated mainly from the proteins present in the malt and, to a minor extent, from the mannoproteins in brewer's yeast cell walls. Here, we describe the isolation and characterization of the novel fermentation gene CFG1 (Carlsbergensis foaming gene) from Saccharomyces pastorianus. CFG1 encodes the cell wall protein Cfg1p, a 105 kDa protein highly homologous to Saccharomyces cerevisiae cell wall mannoproteins, particularly those involved in foam formation, such as Awa1p and Fpg1p. Further characterization of Cfg1p revealed that this novel protein is responsible for beer foam stabilization. This report represents the first time that a brewing yeast foaming gene has been cloned and its action fully characterized.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf3027974