Cell membrane electropermeabilization with arbitrary pulse waveforms

We present a detailed design of a system for in vitro electropermeabilization with arbitrary waveforms. A low-voltage signal is generated by a programmable function generator and amplified by a bipolar amplifier circuit built from commercial components. We describe the general outline of the setup,...

Full description

Saved in:
Bibliographic Details
Published in:IEEE engineering in medicine and biology magazine 2003-01, Vol.22 (1), p.77-81
Main Authors: Flisar, K., Puc, M., Kotnik, T., Miklavcic, D.
Format: Article
Language:English
Subjects:
Amplifiers
Amplifiers, Electronic
Animals
Biomembranes
Cell Membrane - physiology
Cell Membrane - radiation effects
Cell Membrane Permeability - physiology
Cell Membrane Permeability - radiation effects
Cell Survival - physiology
Cell Survival - radiation effects
Cells (biology)
Cells, Cultured
CHO Cells - physiology
CHO Cells - radiation effects
Circuit design
Circuits
Cricetinae
Design engineering
Devices
Electric circuits
Electromagnetic Fields
Electroporation - instrumentation
Electroporation - methods
Equipment Design
Fibroblasts - physiology
Fibroblasts - radiation effects
Frequency
Function generators
In vitro
Pulse amplifiers
Pulse shaping methods
Shape
Signal generators
Studies
Voltage
Waveforms
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We present a detailed design of a system for in vitro electropermeabilization with arbitrary waveforms. A low-voltage signal is generated by a programmable function generator and amplified by a bipolar amplifier circuit built from commercial components. We describe the general outline of the setup, give the scheme of the amplifier circuit, and present the frequency characteristics of the system. Unlike the commercially available devices used for electropermeabilization, the system presented in this article provides a custom choice of the pulse waveform, with the amplitude from 0 up to 260 V (520 V peak-to-peak) with a shape distortion below 5% for the band from 500 Hz up to 35 kHz, and below 15% up to 55 kHz. The circuit can deliver currents up to 5.2 A, which, at the maximum output voltage, is obtained on a resistive load of 50 W. For larger loads (lower resistivity), the performance of the circuit is reduced, with a possibility of malfunction. The total cost of the amplifier circuit components is less than US400, and with programmable function generators starting at approximately US1,000, this makes the presented design attainable to any laboratory with interest in electropermeabilization.
ISSN:0739-5175
1937-4186
DOI:10.1109/MEMB.2003.1191453