Nanosurface design of dental implants for improved cell growth and function

A strategy was proposed for the topological design of dental implants based on an in vitro survey of optimized nanodot structures. An in vitro survey was performed using nanodot arrays with dot diameters ranging from 10 to 200 nm. MG63 osteoblasts were seeded on nanodot arrays and cultured for 3 day...

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Bibliographic Details
Published in:Nanotechnology 2012-08, Vol.23 (33), p.335703-335703
Main Authors: Pan, Hsu-An, Hung, Yao-Ching, Chiou, Jin-Chern, Tai, Shih-Ming, Chen, Hsin-Hung, Steven Huang, G
Format: Article
Language:English
Subjects:
Analysis of Variance
Calcification, Physiologic
Calcium - metabolism
Cell Line, Tumor
Cell Physiological Phenomena - drug effects
Cytoskeleton - drug effects
Dental Implants
Focal Adhesions - drug effects
Humans
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
Osteoblasts - cytology
Osteoblasts - metabolism
Osteoblasts - physiology
Phosphates - metabolism
Tissue Engineering
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Summary:A strategy was proposed for the topological design of dental implants based on an in vitro survey of optimized nanodot structures. An in vitro survey was performed using nanodot arrays with dot diameters ranging from 10 to 200 nm. MG63 osteoblasts were seeded on nanodot arrays and cultured for 3 days. Cell number, percentage undergoing apoptotic-like cell death, cell adhesion and cytoskeletal organization were evaluated. Nanodots with a diameter of approximately 50 nm enhanced cell number by 44%, minimized apoptotic-like cell death to 2.7%, promoted a 30% increase in microfilament bundles and maximized cell adhesion with a 73% increase in focal adhesions. An enhancement of about 50% in mineralization was observed, determined by von Kossa staining and by Alizarin Red S staining. Therefore, we provide a complete range of nanosurfaces for growing osteoblasts to discriminate their nanoscale environment. Nanodot arrays present an opportunity to positively and negatively modulate cell behavior and maturation. Our results suggest a topological approach which is beneficial for the design of dental implants.
ISSN:0957-4484
1361-6528
DOI:10.1088/0957-4484/23/33/335703