Biocompatible Microfabrication of 3D Isolation Chambers for Targeted Confinement of Individual Cells and Their Progeny

We describe a technique to physically isolate single/individual cells from their surrounding environment by fabricating three-dimensional microchambers around selected cells under biocompatible conditions. Isolation of targeted cells is achieved via rapid fabrication of protein hydrogels from a bioc...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-11, Vol.84 (21), p.8985-8989
Main Authors: Harper, Jason C, Brozik, Susan M, Brinker, C. Jeffrey, Kaehr, Bryan
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Applied sciences
Bacteria
Biocompatible Materials
Cell Separation - methods
Cell Survival
Cells
Cellular biology
Chemistry
Escherichia coli - cytology
Exact sciences and technology
Global environmental pollution
Hydrogels
Microtechnology - methods
Pollution
Proteins
Saccharomyces cerevisiae - cytology
Serum Albumin, Bovine - chemistry
Staphylococcus aureus - cytology
Three dimensional imaging
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Summary:We describe a technique to physically isolate single/individual cells from their surrounding environment by fabricating three-dimensional microchambers around selected cells under biocompatible conditions. Isolation of targeted cells is achieved via rapid fabrication of protein hydrogels from a biocompatible precursor solution using multiphoton lithography, an intrinsically 3D laser direct write microfabrication technique. Cells remain chemically accessible to environmental cues enabling their propagation into well-defined, high density populations. We demonstrate this methodology on gram negative (E. coli), gram positive (S. aureus), and eukaryotic (S. cerevisiae) cells. The opportunities to confine viable, single/individual-cells and small populations within user-defined microenvironments afforded by this approach should facilitate the study of cell behaviors across multiple generations.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac301816c