Passive and active targeting of quantum dots for whole-body fluorescence imaging of breast cancer xenografts

Far‐red and near‐infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole‐body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with pol...

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Bibliographic Details
Published in:Journal of biophotonics 2012-11, Vol.5 (11-12), p.860-867
Main Authors: Balalaeva, Irina V., Zdobnova, Tatiana A., Krutova, Irina V., Brilkina, Anna A., Lebedenko, Ekaterina N., Deyev, Sergey M.
Format: Article
Language:English
Subjects:
Animals
breast cancer
Breast Neoplasms - diagnosis
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cell Line, Tumor
Cell Transformation, Neoplastic
CHO Cells
Cricetinae
Cricetulus
Female
fluorescence
HER2/neu
Humans
Mice
Mice, Nude
Molecular Probes - chemistry
Molecular Probes - metabolism
Quantum Dots
Receptor, ErbB-2 - metabolism
scFv antibodies
Spectrometry, Fluorescence
Whole Body Imaging - methods
whole-body imaging
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Summary:Far‐red and near‐infrared fluorescent quantum dots (QDs) have become advancing contrast agents for efficient whole‐body tumor imaging. In this study, we investigated the possibility of the vital fluorescence imaging of tumor using two contrast agents on the basis of QDs: bioinert QDs coated with polyethyleneglycol and QDs bound with anti‐HER2/neu scFv antibodies. HER2/neu‐positive breast cancer tumor xenografts in nude mice were used as a model. It was shown that both bioinert and tumor‐targeted QD probes can be successfully applied for visualization of the tumor using in vivo imaging method, but fluorescent signal of QD‐4D5scFv in tumors was considerably stronger than that of QD‐PEG. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201200080