Effect of alternate day feeding strategy of sub‐optimal protein level on haemato‐biochemical responses in Labeo rohita (Hamilton) juveniles

• Published in: Journal of animal physiology and animal nutrition 2012-12, Vol.96 (6), p.1012-1019
• Main Authors: Kumar, P, Jain, K. K, Munilkumar, S, Sahu, N. P, Siddaiah, G. M, Saha, H
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animal Husbandry; Animal Nutritional Physiological Phenomena; Animals; blood; Blood Glucose; body weight; crude protein; Cyprinidae - blood; Cyprinidae - physiology; Diet - veterinary; dietary protein; Dietary Proteins - analysis; Dietary Proteins - pharmacology; dieting; erythrocyte count; feeding management; fingerlings; fish feeding; haematology; hemoglobin; immunity; ingredients; juveniles; Labeo rohita; phagocytes; Respiratory Burst; serum protein; Time Factors
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/j.1439-0396.2011.01215.x

A feeding trial was conducted for 60 days to delineate the effect of alternate day feeding strategy of sub‐optimal protein level on haematological parameters, serum parameters and phagocyte respiratory burst activity (NBT) in Labeo rohita juveniles. One hundred and thirty‐five fingerlings (1.87 ± 0.01–2.26 ± 0.05 g) were distributed in triplicate groups of each treatment, and fish were fed at 5% body weight daily. Three experimental isocaloric (401.32–410.28 kcal/100 g) diets of 30%, 25%, and 20% crude protein designated as diet A, diet B, and diet C respectively, were prepared, using locally available feed ingredients. Three different feeding schedules of normal protein diet continuously (diet A‐30%), alternate feeding of 1‐day diet A followed by 1‐day diet B (1A/1B) and alternate feeding of 1‐day diet A followed by 1‐day diet C (1A/1C) were tested. The total erythrocyte count and haemoglobin content was significantly (p