Release of acetylcholine from murine embryonic stem cells: Effect of nicotinic and muscarinic receptors and blockade of organic cation transporter

The non-neuronal cholinergic system is widely expressed in nature. The present experiments were performed to characterize the non-neuronal cholinergic system in murine embryonic stem cells (CGR8 cell line). CGR8 cells were cultured in gelatinized flasks with Glasgow's buffered minimal essential...

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Bibliographic Details
Published in:Life sciences (1973) 2012-11, Vol.91 (21-22), p.973-976
Main Authors: Wessler, Ignaz, Michel-Schmidt, Rosmarie, Dohle, Eva, Kirkpatrick, Charles James
Format: Article
Language:English
Subjects:
acetylcholine
Acetylcholine - metabolism
Animals
bioreactors
Cation Transport Proteins - antagonists & inhibitors
Cell Line
CGR8 cell line
choline acetyltransferase
cholinesterase
Cholinesterase Inhibitors - pharmacology
Cholinesterases - metabolism
cultured cells
electrochemistry
embryonic stem cells
Embryonic Stem Cells - metabolism
Fractional release rate
gelatinization
high performance liquid chromatography
homeostasis
Mice
Murine embryonic stem cells
Muscarinic Agonists - pharmacology
Muscarinic Antagonists - pharmacology
nicotine
Nicotinic and muscarinic receptors
Nicotinic Antagonists - pharmacology
Non-neuronal acetylcholine
Organic cation transporter
Oxotremorine - pharmacology
physostigmine
Physostigmine - pharmacology
quinine
Quinine - pharmacology
receptors
Receptors, Muscarinic - metabolism
Receptors, Nicotinic - metabolism
transporters
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Summary:The non-neuronal cholinergic system is widely expressed in nature. The present experiments were performed to characterize the non-neuronal cholinergic system in murine embryonic stem cells (CGR8 cell line). CGR8 cells were cultured in gelatinized flasks with Glasgow's buffered minimal essential medium (Gibco, Germany). Acetylcholine was measured by HPLC combined with bioreactor and electrochemical detection. CGR8 cells contained 1.08±0.12pmol acetylcholine/106 cells (n=7) which was reduced to 0.50±0.06pmol/106 cells (n=6; p
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2012.04.020