Microsatellite primers for an Amazonian lowland tropical tree, Protium subserratum (Burseraceae)

• Premise of the study: The first microsatellite primers were developed for Protium subserratum, a widespread Amazonian tree, to investigate genetic differentiation between populations found on clay, brown-sand, and white-sand soils.• Methods and Results: Seventeen primer pairs were identified from...

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Bibliographic Details
Published in:American journal of botany 2012-11, Vol.99 (11), p.e465-e467
Main Authors: Misiewicz, Tracy M, Barbosa, Carlos E. A, Fine, Paul V. A
Format: Article
Language:English
Subjects:
alleles
Amazon
Biomarkers
Botany
Burseraceae
Burseraceae - genetics
Burseraceae - growth & development
clay
DNA Primers - genetics
DNA, Plant - chemistry
DNA, Plant - genetics
Ecosystem
gene flow
Gene loci
Genetic diversity
Genetic Variation
Genotype
Habitats
loci
microsatellite
microsatellite repeats
Microsatellite Repeats - genetics
Molecular Sequence Data
Polymorphism, Genetic
Protium subserratum
Sequence Analysis, DNA
Soil - analysis
soil types
Trees
Trees - genetics
Trees - growth & development
Tropical Climate
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Summary:• Premise of the study: The first microsatellite primers were developed for Protium subserratum, a widespread Amazonian tree, to investigate genetic differentiation between populations found on clay, brown-sand, and white-sand soils.• Methods and Results: Seventeen primer pairs were identified from two individuals of P. subserratum found on white-sand and brown-sand soil types. Polymorphism was analyzed in 63 individuals from a total of three populations, each found on a different soil type. The primers amplified tetra-, tri-, and dinucleotide repeats with three to 24 alleles per locus. Excluding monomorphic loci, observed and expected heterozygosities ranged from 0 to 0.852 and 0.036 to 0.901, respectively.• Conclusions: These new microsatellite markers will be useful in studies of genetic diversity, population differentiation, and gene flow across habitat types in P. subserratum.
ISSN:0002-9122
1537-2197
DOI:10.3732/ajb.1200229