Development of an integrated electrochemical system for in vitro yeast viability testing

This work describes the development and testing of a microfabricated sensor for rapid cell growth monitoring, especially focused on yeast quality assessment for wine applications. The device consists of a NMOS ISFET sensor with Si3N4 gate, able to indirectly monitor extracellular metabolism through...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2013-02, Vol.40 (1), p.315-322
Main Authors: Adami, Andrea, Ress, Cristina, Collini, Cristian, Pedrotti, Severino, Lorenzelli, Leandro
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensors
Biotechnology
Cell growth monitoring
Conductometry - instrumentation
Electrochemistry - instrumentation
Equipment Design
Equipment Failure Analysis
Food Analysis - instrumentation
Fundamental and applied biological sciences. Psychology
Integrated reference electrode
ISFET
Methods. Procedures. Technologies
Reproducibility of Results
Saccharomyces cerevisiae - isolation & purification
Sensitivity and Specificity
Systems Integration
Transistors, Electronic
Various methods and equipments
Wine - analysis
Wine - microbiology
Wine yeast
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Summary:This work describes the development and testing of a microfabricated sensor for rapid cell growth monitoring, especially focused on yeast quality assessment for wine applications. The device consists of a NMOS ISFET sensor with Si3N4 gate, able to indirectly monitor extracellular metabolism through pH variation of the medium, and a solid-state reference electrode implemented with PVC membranes doped with lipophilic salts (tetrabutylammonium-tetrabutylborate (TBA-TBB) and Potassium tetrakis(4-chlorphenyl)borate (KTClpB)). The use of a solid state reference electrode enables the implementation of a large number of cell assays in parallel, without the need of external conventional reference electrodes. Microbial growth testing has been performed both in standard culture conditions and on chip at different concentrations of ethanol in order to carry out a commonly used screening of wine yeast strains. Cell growth tests can be performed in few hours, providing a fast, sensitive and low cost analysis with respect to the conventional procedures.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2012.07.070