Rapid determination of polycyclic aromatic hydrocarbons (PAHs) in tea using two-dimensional gas chromatography coupled with time of flight mass spectrometry

• Published in: Talanta (Oxford) 2012-10, Vol.100, p.207-216
• Main Authors: Drabova, Lucie, Pulkrabova, Jana, Kalachova, Kamila, Tomaniova, Monika, Kocourek, Vladimir, Hajslova, Jana
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Cost-Benefit Analysis; Dried tea leaves; Exact sciences and technology; Food Analysis - economics; Food Analysis - methods; Food Contamination - analysis; Gas chromatographic methods; Gas Chromatography-Mass Spectrometry - economics; Gas Chromatography-Mass Spectrometry - methods; GC×GC–TOFMS; MIPs; Molecular Imprinting; Molecularly imprinted polymers; PAHs; Polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - isolation & purification; Polymers - chemical synthesis; Safety; Solid Phase Extraction; Solvents - chemistry; Spectrometric and optical methods; Tea - chemistry; Time Factors
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.07.081

A simple, fast, and cost effective sample preparation procedure has been developed and validated for the determination of 15+1 European Union Polycyclic Aromatic Hydrocarbons (15+1 EU PAHs) in dried tea leave samples. Based on a critical assessment of several sample extraction/clean-up approaches, the method based on the ethyl acetate extraction followed by the use of PAHs dedicated cartridges with molecularly imprinted polymers (MIPs) has been found as an optimal alternative in terms of time demands and obtained good extract purity. For the final identification/quantification of target PAHs, two dimensional gas chromatography coupled to a time-of-flight mass spectrometry (GC×GC–TOFMS) was used. The performance characteristics of the overall analytical method for individual PAHs determined at three spiking levels (0.5, 2.5 and 5μgkg−1) were in following ranges: limits of quantitation (LOQs) 0.05–0.2μgkg−1, repeatabilities 2–9%, and recoveries 73–103%. The recoveries achieved by the newly developed sample preparation procedure when employed for naturally contaminated sample (“incurred” PAHs) were comparable to those obtained by other routinely used approaches employing sonication and/or pressurised liquid extraction for sample analytes isolation. The validated method was subsequently used for the determination of selected genotoxic PAHs in 36 samples of black and green tea obtained from the Czech retail market. The levels of ΣPAH4 (sum of benzo[a]anthracene (BaA), chrysene (CHR), benzo[b]fluoranthene (BbFA) and benzo[a]pyrene (BaP)) in black and green tea leaves ranged from 7.4 to 700μgkg−1 and from 4.5 to 102μgkg−1, respectively. Contamination of tested tea samples by BaP was in the range of 0.2–152μgkg−1. ► New rapid procedure for analysis of 15+1 EU PAHs in dried tea leaves was developed. ► Ethyl acetate extraction followed by clean-up using SupelMIP™ was used. ► GC×GC–TOFMS enabled satisfactory separation/quantification of critical PAH groups.