Virtual electrochemical nitric oxide analyzer using copper, zinc superoxide dismutase immobilized on carbon nanotubes in polypyrrole matrix

• Published in: Talanta (Oxford) 2012-10, Vol.100, p.168-174
• Main Authors: Madasamy, Thangamuthu, Pandiaraj, Manickam, Balamurugan, Murugesan, Karnewar, Santosh, Benjamin, Alby Robson, Venkatesh, Krishna Arun, Vairamani, Kanagavel, Kotamraju, Srigiridhar, Karunakaran, Chandran
• Format: Article
• Language: English
• Subjects: Adult; Analytical chemistry; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Breath Tests; Chemistry; Copper, zinc superoxide dismutase; Electrochemical methods; Electrochemical nitric oxide analyzer; Electrochemistry; Electrodes; Endothelial cells; Endothelial Cells - drug effects; Endothelial Cells - secretion; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Exact sciences and technology; Exhalation; Exhaled breath; Fundamental and applied biological sciences. Psychology; General, instrumentation; Home-made potentiostat; Humans; Hydrogen Peroxide - pharmacology; Hydrogen-Ion Concentration; Methods. Procedures. Technologies; Nanotubes, Carbon - chemistry; Nitric Oxide - metabolism; Nitric Oxide - secretion; Platinum - chemistry; Polymers - chemistry; Programming Languages; Pyrroles - chemistry; Superoxide Dismutase - chemistry; Superoxide Dismutase - metabolism; Various methods and equipments; Virtual instrument
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.08.033

In this work, we have designed and developed a novel and cost effective virtual electrochemical analyzer for the measurement of NO in exhaled breath and from hydrogen peroxide stimulated endothelial cells using home-made potentiostat. Here, data acquisition system (NI MyDAQ) was used to acquire the data from the electrochemical oxidation of NO mediated by copper, zinc superoxide dismutase (Cu,ZnSOD). The electrochemical control programs (graphical user-interface software) were developed using LabVIEW 10.0 to sweep the potential, acquire the current response and process the acquired current signal. The Cu,ZnSOD (SOD1) immobilized on the carbon nanotubes in polypyrrole modified platinum electrode was used as the NO biosensor. The electrochemical behavior of the SOD1 modified electrode exhibited the characteristic quasi-reversible redox peak at the potential, +0.06V vs. Ag/AgCl. The biological interferences were eliminated by nafion coated SOD1 electrode and then NO was measured selectively. Further, this biosensor showed a wide linear range of response over the concentration of NO from 0.1μM to 1mM with a detection limit of 0.1μM and high sensitivity of 1.1μAμM−1. The electroanalytical results obtained here using the developed virtual electrochemical instrument were also compared with the standard cyclic voltammetry instrument and found in agreement with each other. ► A novel virtual electrochemical nitric oxide analyzer using Cu, ZnSOD developed. ► Highly flexible electrochemical control programs were developed using LabVIEW 10.0. ► It has been applied to measure the NO level present in the biological samples.