Circulating miRNA is a novel marker for head and neck squamous cell carcinoma

• Published in: Tumor biology 2012-12, Vol.33 (6), p.1933-1942
• Main Authors: Hsu, Cheng-Ming, Lin, Pai-Mei, Wang, Yu-Ming, Chen, Zong-Jyun, Lin, Sheng-Fung, Yang, Ming-Yu
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Biomedical and Life Sciences; Biomedicine; Cancer Research; Carcinoma, Squamous Cell - blood; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Case-Control Studies; Female; Gene Expression Regulation, Neoplastic; Head & neck cancer; Head and Neck Neoplasms - blood; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - pathology; Humans; Male; MicroRNAs - blood; MicroRNAs - genetics; Middle Aged; Neoplasm Staging; Polymerase chain reaction; Prognosis; Real-Time Polymerase Chain Reaction; Research Article; Reverse Transcriptase Polymerase Chain Reaction; Ribonucleic acid; RNA
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-012-0454-8

The aim of the study is to investigate the alteration of plasma miRNA in head and neck squamous cell carcinoma (HNSCC). Altered microRNAs (miRNAs) expression has been found in many cancers, including lung cancer, breast cancer, prostate cancer, bladder cancer and colorectal cancer. Many recent studies have demonstrated that aberrant plasma miRNAs were also found in various types of cancers. However the alteration of plasma expression in HNSCC remains unclear. In this present study, the expression profiles of ten miRNAs, let-7a , miR-21 , miR26b , miR-34c , miR-99a , miR-133a , miR-137 , miR-184 , miR-194a , and miR-37 5, in plasma from 50 patients and 36 healthy subjects were evaluated using real-time quantitative polymerase chain reaction (PCR). Our results demonstrated that the expression level of miR-21 was significantly up-regulated in plasma samples obtained from HNSCC patients ( p